GEO DataSets

Analysis of Trp53-null tumors arising from Tgfb1+/- hosts cleared of mammary gland epithelia, irradiated, then transplanted with nonmalignant Trp53-null mammary tissue. TGF-β drives EMT and is highly induced by IR. Results provide insight into host biology contributions to radiation carcinogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL1261

Series:

GSE42742

24 Samples

Download data: CEL

(Submitter supplied) We developed a mouse model that captures radiation effects on host biology by transplanting unirradiated Trp53 null mammary tissue to sham or irradiated hosts. Gene expression profiles of tumors that arose in irradiated mice are distinct from those that arose in naïve hosts. Host irradiation induces a metaprofile consisting of gene modules representing stem cells, cell motility, macrophages and autophagy. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4768 GDS4769

Platform:

GPL1261

56 Samples

Download data: CEL

Analysis of Trp53-null mammary tumors arising from WT hosts cleared of mammary gland epithelium, irradiated, then transplanted with nonmalignant Trp53-null mammary tissue. Results provide insight into the influence of the radiation response of the microenvironment on the carcinogenic process.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL1261

Series:

GSE42742

32 Samples

Download data: CEL

(Submitter supplied) Densely ionizing radiation is a major component of the space radiation environment and has potentially greater carcinogenic effect compared to sparsely ionizing radiation that is prevalent in the terrestrial environment. It is unknown to what extent the irradiated microenvironment contributes to the differential carcinogenic potential of densely ionizing radiation. To address this gap, 10-week old BALB/c mice were irradiated with 100 cGy sparsely ionizing g-radiation or 10, 30, or 80 cGy of densely ionizing, 350 MeV/amu Si particles and transplanted 3 days later with syngeneic Trp53 null mammary fragments. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11533

45 Samples

Download data: CEL

(Submitter supplied) It is widely believed that the carcinogenic action of ionizing radiation is due to targeted DNA damage and resulting mutations, but there is also substantial evidence that non-targeted radiation effects alter epithelial phenotype and the stromal microenvironment. Activation of transforming growth factor β1 (TGFβ) is a non-targeted radiation effect that mediates cell fate decisions following DNA damage and regulates microenvironment composition; it could either suppress or promote cancer. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

65 Samples

Download data: CEL

(Submitter supplied) Irradiated hosts gave rise to significantly more Trp53 null mammary cancers that grew more rapidly than those in sham-irradiated mice and exhibited an immunosuppressive tumor microenvironment . CAPE prevented the effect of host irradiation on tumor growth rate, immune signature and immunosuppression.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

23 Samples

Download data: TXT

(Submitter supplied) To determine how host irradiation affects tumor profiles in 10 month aged mice treated with HZE or gamma irradiation.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

43 Samples

Download data: CEL

(Submitter supplied) The claudin-low subtype is a recently identified rare molecular subtype of human breast cancer that expresses low levels of tight and adherens junction genes and shows high expression of epithelial-to-mesenchymal transition (EMT) genes. These tumors are enriched in gene expression signatures derived from human tumor initiating cells (TIC) and human mammary stem cells. Through cross-species analysis, we discovered mouse mammary tumors that have similar gene expression characteristics as human claudin-low tumors and were also enriched for the human TIC signature. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Genome variation profiling by array; Genome variation profiling by genome tiling array

Platforms:

GPL10732 GPL4092 GPL4134

107 Samples

Download data

(Submitter supplied) WAP-Cre:Ptenf/f:p53lox.stop.lox_R270H composite mice were generated by genetic crossing. In these mice, Pten is deleted and a R270H p53 mutation in the DNA binding domain is induced upon expression of Cre recombinase in pregnancy-identified alveolar progenitors. Tumors were characterized by histology, marker analysis, various bioinformatics methods, high-throughput (HTP) FDA-drug screen as well as orthotopic injection to quantify tumor initiating cells (TICs) and tail-vein injection to identify lung-metastasis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

31 Samples

Download data: CEL

(Submitter supplied) Age and physiological status like menopause are key factors in mammary development and are associated with breast cancer risk. Less clear is what factors influence breast cancer intrinsic subtypes that are associated with prognosis. Here, we investigated the age of the host in a mammary chimera model. The mammary glands of wildtype BALB/c mice were cleared of endogenous epithelium at 3 weeks of age and subsequently transplanted during puberty (5 weeks) or upon maturation (10 weeks) with syngeneic Trp53null mammary fragments. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

27 Samples

Download data: TXT

(Submitter supplied) Breast cancer is the second leading cause of cancer related death in American women. Patient care is complicated by inherent tumor heterogeneity that can be classified into at least six intrinsic subtypes. While targeted treatments are standard of care for most subtypes, there remains a clinical need for targeted therapies against basal-like tumors that are typically ‘triple negative breast cancers’. more...

Organism:

Mus musculus

Type:

Genome variation profiling by genome tiling array

Platform:

GPL4092

5 Samples

Download data: TXT

(Submitter supplied) CNV profiling of tumors obtained from our Chaos3 mouse model for spontaneous breast cancer. The goal of this experiment was to determine copy number variations that were specific to MTs derived from this mouse model, when comapared to non-MTs.

Organism:

Mus musculus

Type:

Genome variation profiling by genome tiling array

Platform:

GPL10448

15 Samples

Download data: TXT

(Submitter supplied) The goal of this study was to identify important genetic pathways that are altered in mammary tumor cells upon over-expression of the tumor suppressor gene Arid1a. The results of this experiment revealed that Arid1a helps regulate key cell-cycle checkpoint and growth regulatory pathways, either directly or indirectly. This helped explain in part the significant decrease in cell proliferation and tumor growth phenotypes observed both in vitro and in vivo, when comparing the same samples analyzed here by RNA-seq (untransduced replicates vs. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: DIFF, TXT

(Submitter supplied) Introduction: A gene expression signature indicative of active wound responses is common to more than 90% of non-neoplastic tissues adjacent to breast cancer, but these tissues also show substantial heterogeneity, suggesting different microenvironmental subtypes. Genomic variation in the extratumoral microenvironment has received limited study. Methods: Gene expression analysis from 72 patient-derived samples adjacent to invasive breast cancer or ductal carcinoma in situ was evaluated. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

5 related Platforms

74 Samples

Download data

(Submitter supplied) Loss of E2F transcription factos alters metastatic capacity of MMTV-PyMT tumors. We used microarrays to futher characterize the effects of E2F loss on mammary tumorigenesis in MMTV-PyMT mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

80 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of mammary tissue irradiated at 10 weeks of age with either 100 cGy sparsely ionizing gamma-rays, or 10 cGy or 30 cGy densely ionizing radiation (350 MeV/amu Si). Mammary tissue was collected 1 weeks, 4 weeks, and 12 weeks post-irradiation.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4905

Platform:

GPL11533

37 Samples

Download data: CEL

Analysis of mammary gland from females irradiated at 10 wks of age with varying doses of sparsely or densely ionizing radiation (IR). Mammary tissue collected up to 12 wks post-IR. Results provide insight into molecular response of mammary tissue exposed to different radiation qualities and doses.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 4 dose, 3 stress, 3 time sets

Platform:

GPL11533

Series:

GSE48392

37 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of mammary tumors that occurs in parous, BK5.ATF3 mice, compared to adjacent normal mammary tissue

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of mammary tumors that occur in parous, BK5.ATF3 mice, compared to normal mammary tissue

Organism:

Murid gammaherpesvirus 4; Mus musculus; Murid betaherpesvirus 1

Type:

Non-coding RNA profiling by array

Platform:

GPL29478

7 Samples

Download data: GPR

(Submitter supplied) Macrophages are frequently the most abundant immune cells in murine and human cancers. Studies in various transgenic mouse tumor models have revealed pro-tumor functions of tumor-associated macrophages (TAMs), but despite their association with poor clinical outcome in human patients, molecular signatures for the prediction of clinical outcome in humans are still missingbeen demonstrated. Here we generated molecular signatures from F4/80+CD11b+ TAMs from two transgenic breast cancer models: K14cre;Cdh1flox/flox;Trp53flox/flox (KEP), which resembles human invasive lobular carcinoma (ILC) and MMTV-NeuT (NeuT), which resembles HER2-overexpressing breast cancer. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Third-party reanalysis

Platform:

GPL18480

44 Samples

Download data: TXT, XLSX