GEO DataSets

Analysis of lungs from MyD88 null mutants following Chlamydia pneumoniae infection. Toll-like receptors and key adaptor protein MyD88 play a critical role in inducing immunity against this microorganism. Results provide insight into the molecular mechanisms underlying this MyD88-mediated induction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 infection sets

Platform:

GPL339

Series:

GSE6688

12 Samples

Download data: CEL

(Submitter supplied) This experiment is an additional experiment to GSE6688. Mouse macrophages (ANA-1 cells) were infected in vitro with C. pneumoniae with a M.O.I. of 10. Twenty two genes were significantly upregulated. Examples of the most upregulated genes in mouse macrophages after C. pneumoniae infection are serum amyloid A3 (saa3), a protein that is mainly produced by activated macrophages during tissue injury or inflammation, MIP-2 (cxcl2) and irg1. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2651

Platform:

GPL81

4 Samples

Download data: CEL

(Submitter supplied) Chlamydia pneumoniae, an obligate intracellular bacterium, causes pneumonia in humans and mice. Toll-like receptors and the key adaptor molecule MyD88 play a critical role in inducing immunity against this microorganism and are crucial to survive the infection. To explore the influence of MyD88 on induction of immune responses in vivo on a genome wide level, WT or MyD88-/- mice were infected with C. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2650

Platform:

GPL339

12 Samples

Download data: CEL

Analysis of ANA-1 macrophage cells infected with Chlamydia pneumoniae, an intracellular bacterium that causes pneumonia. Results provide insight into the contribution of the macrophage cell type in host defense against C. pneumoniae.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 infection sets

Platform:

GPL81

Series:

GSE6690

4 Samples

Download data: CEL

(Submitter supplied) TLRs are considered important for innate immune responses that combat bacterial infections. Here, the role of TLRs in severe septic peritonitis using the colon ascendens stent peritonitis (CASP) model was examined. We demonstrate that mice deficient for MyD88 and TRIF had markedly reduced bacterial numbers both in peritoneal cavity and peripheral blood, indicating that bacterial clearance in this model is inhibited by TLR signals. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4433

Platform:

GPL6246

12 Samples

Download data: CEL

Analysis of spleens of MyD88 and TRIF double null mutants following surgical induction of septic peritonitis. MyD88 and TRIF are involved in innate immune responses that combat bacterial infections. Results provide insight into the role of MyD88 and TRIF in the pathogenesis of septic peritonitis.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 disease state, 2 genotype/variation sets

Platform:

GPL6246

Series:

GSE24327

12 Samples

Download data: CEL

(Submitter supplied) Characterization of the zebrafish embryonic host response to systemic bacterial infection with Salmonella typhimurium wild type strain (SL1027) and its isogenic LPS O-antigen mutant Ra (SF1592) by means of a time-resolved global expression analysis.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL7735

36 Samples

Download data: XML

(Submitter supplied) Messenger RNA of C trachomatis infected monocytes of the three healthy donors was compared with the corresponding mock-infected samples. For mock infection, SPG buffer was used instead of the C trachomatis suspension, all other procedures were performed identically. Cells were resuspended in solution D and total RNA was extracted by application of phenol:chloroform 5:1 (pH 4.5, Ambion, Austin, TX) followed by precipitation in isopropanol at -80°C for 1 hr and incubation with RQ1 DNase (Promega, Madison, WI) at 37 °C for 1 hr. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL127

6 Samples

Download data

(Submitter supplied) We wanted to screen the expression of known and putative antichlamydial genes in vivo. As an open approach, we performed RNA sequencing of Chlamydia muriadrum infected mouse lung tissues. RNA seq revealed that a diverse set of antichlamydial genes were induced by the infection, including previously described unique human and murine genes. We further characterized the source and activity of indolamine 2,3-dioxygenase genes, previously described as key human effectors.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16173

6 Samples

Download data: TXT

(Submitter supplied) While recent studies of immunity have uncovered many aspects of the innate immune system, there has been precious little investigation of the cellular response to viruses in vivo. To this end, we exploited high titer adenoviral (Ad) vectors to investigate the cellular response to non-enveloped viral infection in vivo. Our results indicate a potent cellular transcriptome response early after infection, with global assessments revealing significant dysregulation in ~15% of measured transcripts derived from Ad infected tissue. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3222

36 Samples

Download data

(Submitter supplied) Mice were injected intravenously with 1st generation or 2nd generation Ad vectors (each bearing a LacZ transgene) or mock infected (controls) to completely transduce the liver. At different time points post-injection (6 hours, 3 days, and 3 weeks (21 days)), the liver was harvested and the overall transcriptome response to viral infection was assayed to measure the cellular immune response. Keywords: Immune Response

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3223

20 Samples

Download data