GEO DataSets

(Submitter supplied) The lacrimal gland (LG) secretes aqueous tears. Previous studies have provided insights into the cell lineage relationships during tissue morphogenesis. However, little is known about the cell types composing the adult LG and their progenitors. Using scRNA-seq, we established the first comprehensive cell atlas of the adult mouse LG to investigate cell hierarchy, their secretory repertoire and sex differences.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21626 GPL24247

3 Samples

Download data: MTX, TSV

(Submitter supplied) We report sequencing data on fetal mouse submandibular glands (SMGs) where SOX10 was deleted in the epithelia. By obtaining embryonic day 16 (E16) SMGs, we compared control glands with glands where SOX10 was deleted in the epithelial compartment. We found that loss of SOX10 resulted in smaller glands, which had reduced expression of genes related to the initiation of acinar, myoepithelial and intercalated duct cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15103

7 Samples

Download data: TXT

(Submitter supplied) Outputs from scRNA seq reads from isolated Mouse E16 and P4 lacrimal glands

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

2 Samples

Download data: HTML, MTX, TSV

(Submitter supplied) The transcriptional expression analysis of 17.5, 19.5 days embryonic lacrimal gland (LG), adult lacrimal gland, lacrimal gland stem cells (LGSCs) P2 cultured for 5, 7, 10, 14 days and LGSCs P6, P10, P20 cultured for 7 days, respectively.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21273

24 Samples

Download data: TXT

(Submitter supplied) As an element of the lacrimal apparatus, the lacrimal gland (LG) produces the aqueous part of the tear film, which protects the eye surface. Therefore, a defective LG can lead to serious eyesight impairment. Up to now, little is known about LG morphogenesis and subsequent maturation. In this study, we delineated elements of the cellular and molecular events involved in LG formation by using three epithelial markers, namely aSMA, Krt14 andKrt19. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

6 Samples

Download data: CEL, CHP

(Submitter supplied) The mammary glands of adult female mice were divided into ductal tissue and terminal end buds (TEBs). Basal and luminal epithelial cells were FACS sorted. RNA and nuclei were extracted for RNA-seq and ATAC-seq profiling using an Illumina NextSeq 500 sequencer. This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

22 Samples

Download data: TXT

(Submitter supplied) The mammary glands of adult female mice were divided into ductal tissue and terminal end buds (TEBs). Basal and luminal epithelial cells were FACS sorted and nuclei extracted for 75bp paired-end ATAC-seq profiling using an Illumina NextSeq 500 sequencer.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

11 Samples

Download data: BED, TXT

(Submitter supplied) The mammary glands of adult female mice were divided into ductal tissue and terminal end buds (TEBs). Basal and luminal epithelial cells were FACS sorted and RNA extracted for 75bp paired-end RNA-seq profiling using an Illumina NextSeq 500 sequencer.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

11 Samples

Download data: TXT

(Submitter supplied) Background: Heterogeneity within the mouse mammary epithelium has been recently explored by single-cell RNA profiling. To further understand how cellular diversity changes during mammary development, we profiled single cells from different developmental stages including late embryogenesis, early postnatal, prepuberty, resting adult, mid-pregnancy, late-pregnancy, lactation and post-involution. We also compared the transcriptomes and chromatin accessibility of micro-dissected ducts versus terminal endbuds (TEBs) to help decipher the molecular properties of these specialized units that drive morphogenesis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

18 Samples

Download data: MTX, TSV

(Submitter supplied) The organisation of the mammary epithelial cell hierarchy and how these cells relate to the presentation of human breast tumours is poorly understood. Our results demonstrate that the luminal cell compartment of the mouse mammary gland can be resolved into terminally differentiated oestrogen receptor (ER)+ luminal cells, ER+ luminal progenitors and ER- luminal progenitors. The ER+ luminal progenitors are unique in regards to cell survival, as they are relatively insensitive to loss of oestrogen and progesterone when compared to the other types of mammary epithelial cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

72 Samples

Download data: TXT

(Submitter supplied) To identify the transcriptomic alterations within the different cellular compartments of the lacrimal gland during chronic inflammation, we analyzed the lacrimal glands of NOD.B10.H2b vs BALB/cJ with 10X Visium technology

Organism:

Mus musculus

Type:

Other

Platform:

GPL30172

4 Samples

Download data: TAR

(Submitter supplied) To identify the molecular pathways altered during the progression of chronic inflammation in the lacrimal gland, we compared the transcriptome of NOD.B10.H2b vs BALB/cJ mice at different time points

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

18 Samples

Download data: TXT

(Submitter supplied) Through a combination of scRNA-Seq analysis and in-vivo genetic-lineage tracing, we generated a detailed a detailed map of the cell fate trajectories and branch points of the basal and myoepithelial of the mouse SMG during embryonic development and in adults.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

1 Sample

Download data: TXT

(Submitter supplied) Background: Breast cancer arises within specific regions in the human breast referred to as the terminal duct lobular units (TDLUs). These are relatively dynamic structures characterized by sex hormone driven cyclic epithelial turnover. TDLUs consist of unique parenchymal entities embedded within a fibroblast-rich lobular stroma. Here, we established and characterized a new human breast lobular fibroblast cell line against its interlobular counterpart with a view to assessing the role of region-specific stromal cues in the control of TDLU dynamics. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23227

8 Samples

Download data: XLSX

(Submitter supplied) First, we determined the gene expression profile of WT mouse lacrimal gland tissue and organoids in expansion and in differentiation. Then, we sought the differences between WT and Pax6-KO mouse lacrimal gland organoids in expansion and in differentiation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

17 Samples

Download data: CSV

(Submitter supplied) Human lacrimal gland tissue and organoids, cultured in expansion or differentiation, were FAC sorted into plates to follow the SORTseq protocol (Muraro et al., 2016).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

8 Samples

Download data: CSV

(Submitter supplied) Although normal function of the lacrimal gland (LG) is essential for vision (and thus, for human well-being), the LG remains rather poorly understood at molecular level. The purpose of this study was to identify new genes and signaling cascades involved in LG development.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7656

6 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data

(Submitter supplied) Wild type or Fgfr2 fx/fx; Le-Cre-positive (Fgfr2-/-) conjunctival epithelium of E14.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina MouseWG-6 v2.0 bead arrays.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) Wild type or Sox9 fx/fx; Le-Cre-positive (Sox9-/-) conjunctival epithelium from the temporal, nasal and dorsal region of E14.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina MouseWG-6 v2.0 bead arrays.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT