GEO DataSets

(Submitter supplied) Heterochronic blood exchange (HBE) has demonstrated that circulating factors restore youthful features to aged tissues. However, the systemic mediators of those rejuvenating effects remain poorly defined. We show that the beneficial effect of young blood on aged muscle regeneration was diminished when serum was depleted of extracellular vesicles (EVs). Whereas EVs from young animals rejuvenate aged cell bioenergetics and skeletal muscle regeneration, aging shifts EV subpopulation heterogeneity and compromises downstream benefits on recipient cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

11 Samples

Download data: TXT

(Submitter supplied) We have demonstrated that the circulating extracellular vesicles (EVs) are essential to the beneficial effect of young serum on the skeletal muscle regenerative cascade. Here, we show that infusions of young serum significantly improved age-associated memory deficits, and the effect was abolished after serum depletion of EVs. RNA-seq analysis of the choroid plexus demonstrated EV-mediated effects on genes involved in barrier function and trans-barrier transport. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

89 Samples

Download data: XLSX

(Submitter supplied) HeLa cells were treated with ActD and RNA sequenced to examine RNA stability

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

12 Samples

Download data: TXT

(Submitter supplied) Ewing sarcoma shA673-1c cells were treated with ActD and RNA sequenced to examine RNA stability

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

12 Samples

Download data: TXT

(Submitter supplied) RNA was harvested from endothelial cells cultured alone or with tumor cells and the associated endothelial extracellular vesicles.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

14 Samples

Download data: SF

(Submitter supplied) RNA was harvested from endothelial cells treated with VEGF or H2O and their associated extracellular vesicles.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

12 Samples

Download data: SF

(Submitter supplied) RNA was harvested from endothelial cells treated with GW4869, an inhibitor of extracellular vesicle biogenesis

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: SF

(Submitter supplied) In this study, we investigated signaling pathways in Skeletal muscle precursors that are altered with aging and age-related deficits in muscle regenerative potential. We performed fluorescence activated cell sorting (FACS) to obtain highly purified skeletal muscle satellite cells from young, middle-aged and old mice. Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting that young blood contains humoral "rejuvenating" factors that can restore regenerative function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4892

Platform:

GPL1261

14 Samples

Download data: CEL

Analysis of FACS-sorted CD45-Ter119-Sca-1-CD29+Cxcr4+ skeletal muscle precursor (SMP) cells from young, middle-aged, and old C57BL/6 males. Results provide insight into the molecular mechanisms underlying age-related skeletal muscle and stem cell dysfunction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age sets

Platform:

GPL1261

Series:

GSE50821

14 Samples

Download data: CEL