GEO DataSets

(Submitter supplied) Interspecies coaggregation promotes transcriptional changes of oral bacteria, contributing to the development of structurally balanced biofilms as well as oral diseases such as periodontitis. Streptococcus gordonii (S. gordonii) is an early colonizer of the oral cavity, and Fusobacterium nucleatum (F. nucleatum) may act as a bridge adhering to both early and late oral colonizers. These two species were commonly detected in healthy and periodontitis-diseased oral sites and could interact with immune cells such as macrophages. more...

Organism:

Streptococcus gordonii str. Challis substr. CH1; Fusobacterium polymorphum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29567 GPL29569 GPL29568

9 Samples

Download data: FNA, GFF, TXT

(Submitter supplied) Oral streptococci, including Streptococcus gordonii, and Actinomyces naeslundii, are consistently found to be the most abundant bacteria in the early stages of dental plaque accumulation. These organisms interact physically (coaggregate) in vitro and in vivo. We hypothesized that coaggregation between S. gordonii and A. naeslundii leads to changes in gene expression in the partner organisms. Furthermore, we predicted that coaggregation-induced changes in phenotype contribute to the success of streptococci and actinomyces in dental plaque. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL5736

3 Samples

Download data: MEV

(Submitter supplied) A gram-negative colonizer of the oral cavity, Fusobacterium nucleatum not only interacts with many pathogens in the oral microbiome but also has the ability to spread to extraoral sites including placenta and amniotic fluid, promoting preterm birth. To date, however, the molecular mechanism of interspecies interactions –termed coaggregation–by F. nucleatum and how coaggregation affects bacterial virulence remain poorly defined. more...

Organism:

Fusobacterium nucleatum subsp. nucleatum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29799

6 Samples

Download data: XLSX

(Submitter supplied) Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral commensal S. gordonii and the opportunistic commensal F. nucleatum. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Keywords: infection state

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2933

Platform:

GPL96

12 Samples

Download data: CEL

Analysis of gingival epithelial HIGK cells co-cultured with the the oral commensal Streptococcus gordonii or the opportunistic commensal Fusobacterium nucleatum. Results provide insight into the mechanisms underlying the adaptation of hosts to commensals.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 infection sets

Platform:

GPL96

Series:

GSE6927

12 Samples

Download data: CEL

(Submitter supplied) Fusobacterium nucleatum is a Gram negative oral bacterial species associated with periodontal disease progression. This species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. Numerous studies of F. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. more...

Organism:

Fusobacterium nucleatum

Type:

Expression profiling by array

Platform:

GPL9447

6 Samples

Download data: CEL

(Submitter supplied) Recent studies have shown that the transcriptional landscape of the pleiomorphic fungus Candida albicans is highly dependent upon growth conditions. Here using a dual RNA-seq approach we identified 299 C. albicans and 72 Streptococcus gordonii genes that were either up- or down-regulated specifically as a result of co-culturing these human oral cavity microorganisms. Seventy five C. albicans genes involved in responses to chemical stimuli, regulation, homeostasis, protein modification and cell cycle were statistically (P ≤0.05) upregulated, while 36 genes mainly involved in transport and translation were down-regulated. more...

Organism:

Streptococcus gordonii; Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20135 GPL19036 GPL20134

15 Samples

Download data: TXT

(Submitter supplied) Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans and commensal streptococci to assure a state of oral health. To better understand the molecular mechanisms involved in the initial streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii cells for 30 minutes to assess the streptococcal transcriptome response. more...

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL20180

2 Samples

Download data: TXT

(Submitter supplied) Fusobacterium nucleatum, long known as a constituent of the oral microflora, has recently garnered much attention for its newly discovered prevalence in colorectal and breast cancer tissue. The growing interest in this emerging cancer-associated bacterium sharply contrasts with a paucity of knowledge about its basic gene expression features and physiological responses. Post-transcriptional networks are also unknown, for fusobacteria lack all established small RNA-associated proteins. more...

Organism:

Fusobacterium polymorphum ATCC 10953; Fusobacterium animalis 7_1; Fusobacterium vincentii 3_1_36A2; Fusobacterium nucleatum subsp. nucleatum ATCC 25586; Fusobacterium periodonticum 2_1_31

Type:

Expression profiling by high throughput sequencing

5 related Platforms

90 Samples

Download data: WIG

(Submitter supplied) Tn-Seq was used to identify P. gingivalis genes that confer fitness during cooperative growth with S. gordonii or F. nucleatum in a murine abscess model.

Organism:

Porphyromonas gingivalis

Type:

Other

Platform:

GPL28580

20 Samples

Download data: TXT

(Submitter supplied) The mechanisms through which oral commensal bacteria mitigates uncontrolled inflammatory responses in the oral mucosa remain unknown. Here we evaluated the ability of S. gordonii to stimulate the expression of miRNAs in oral epithelial cells with potential to target chemokine expression. The human oral epithelial cell line (OKF6) was exposed to different MOIs of S. gordonii for 24h and expression analysis of miRNAs performed using the Affymetrix platform.

Organism:

synthetic construct; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

8 Samples

Download data: CEL

(Submitter supplied) To better understand the global responses of GFs to F. nucleatum infection along the time sequence, we performed a genome-wide transcriptome analysis by RNA-sequencing technology to determine the global changes on gene expression level

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23227

54 Samples

Download data: TXT