GEO DataSets

(Submitter supplied) Streptococcus mutans, the organism most frequently associated with the development of dental caries, is able to utilize a diverse array of carbohydrates for energy metabolism. One such molecule is trehalose, a disaccharide common in human foods, which has recently been implicated in enhancing the virulence of epidemic strains of the pathogen, Clostridium difficile. In this study, deletion mutants of all three genes in the putative S. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24591

18 Samples

Download data: TXT, XLSX

(Submitter supplied) The glucose/mannose-PTS permease EIIMan encoded by manLMN in the dental caries pathogen Streptococcus mutans has a dominant influence on sugar-specific, CcpA-independent catabolite repression (CR). Mutations in manL affect energy metabolism and virulence-associated traits, including biofilm formation, acid tolerance and competence. Using promoter:reporter fusions, expression of the manLMN and the fruRKI operons, encoding a transcriptional regulator, a fructose-1-P kinase and a fructose-PTS permease EIIFru, respectively, was monitored in response to carbohydrate source and in mutants lacking CcpA, FruR, and components of EIIMan. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

9 Samples

Download data: XLSX

(Submitter supplied) H2O2 is an oxidative stress agent known to trigger an upregulation in expression of detoxification genes in Streptococcus mutans. Following discovery of a spontaneously-occurring perR mutation, we wanted to compare the transcriptomic responses of H2O2 exposure in S. mutans strains bearing an intact perR or a perR deletion.

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29159

12 Samples

Download data: CSV, XLSX

(Submitter supplied) In this study we analyzed the transcriptional regulation of the operon (OBio) encoding the PTSBio and showed that it was repressed by NigR, a LacI-like transcriptional regulator. To elucidate the role of NigR in regulation of S. mutans carbohydrate transporters we performed full genome expression microarrays using custom Affymetrix chip. Expression microarray analysis was performed with ΔnigR grown in CDM supplemented with glucose and compared to that of WT grown under identical conditions. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4769

4 Samples

Download data: CEL, CHP

(Submitter supplied) Iron and manganese are micronutrientients that serve as important co-factors necessary for enzymatic function. In the host environment, access to metals can be highy restricted. Here, we wanted to identify the transcriptomic response when S. mutans UA159 is grown in media depleted of iron, manganese, or both.

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

12 Samples

Download data: CSV

(Submitter supplied) Streptococcus mutans, the primary etiologic agent of human dental caries, and a variety of oral Streptococcus and Actinomyces spp. synthesize high molecular mass homopolymers of fructose (fructans) with predominantly β2,1- (inulins) or β2,6-linkages (levans). The ability of S. mutans to degrade fructans contributes to the severity of dental caries. The extracellular product of fruA of S. mutans is an exo-β-D-fructofuranosidase that releases fructose from levan and inulin. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

6 Samples

Download data: CSV

(Submitter supplied) Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In this study, we observed supplementation with 1.5% arginine (final concentration) had inhibitory effects on the growth of S. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

18 Samples

Download data: XLSX

(Submitter supplied) S. mutans UA159 was cultivated in chemically defined medium. Changes in the transcriptome in response to the autoinducing peptides CSP and XIP were monitored for 30 minutes.

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19789

20 Samples

Download data: TXT

(Submitter supplied) Recently, we described the function of a novel two-gene regulatory system consisting of a LytTR family transcription regulator and a membrane protein, which we referred to as the hdrRM operon. We determined that the HdrRM system controls the expression of another similar regulatory system annotated as SMU.2080 and SMU.2081. Like hdrRM, the SMU.2080 – 2081 operon encodes a LytTR family transcription regulator and membrane protein, which we now refer to as BrsR and BrsM, respectively. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4769

2 Samples

Download data: CEL

(Submitter supplied) CcpA is a global regulator of transcription in Gram-positive bacteria that controls gene expression in response to carbohydrate availability. Using the fructan hydrolase (fruA) gene of S. mutans as a model, we demonstrate that CcpA does indeed play a major role in carbohydrate catabolite repression. Using DNA microarrays, the expression of at least 170 genes differed in CcpA-deficient cells compared to the parental strains when cells are grown in the presence of glucose, but only 90 genes showed altered expression when cells were cultivated in the poorly-repressing substrate galactose. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4340

16 Samples

Download data: MEV

(Submitter supplied) Transcriptional analysis of the effects of oxygen concentraion in S. mutans

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL9277

12 Samples

Download data: MEV

(Submitter supplied) Bacteria can detect, transmit and react to signals from the outside world by using two-component systems an serine-threonine kinases and phosphatases. Streptococcus mutans contains one serine-threonine kinase, encoded by pknB. A gene encoding a serine-threonine phosphatase, pppL, is located downstream of pknB. In this study, the phenotypes of single mutants in pknB and pppL and a pknBpppL double mutant were characterized. more...

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL9332

3 Samples

Download data: TXT

(Submitter supplied) Global transcriptional analysis of acid-inducible genes in Streptococcus mutans: multiple two-component systems involved in acid adaptation pH is a major environmental factor that regulates gene expression in many bacteria. Streptococcus mutans in dental biofilms is regularly exposed to cycles of acidic pH during the ingestion of fermentable dietary carbohydrates. The ability of S. mutans to tolerate low pH is crucial for its virulence and the pathogenesis in dental caries. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL8255

3 Samples

Download data: TXT

(Submitter supplied) The induction of genetic competence is a strategy used by bacteria to increase their genetic repertoire under stressful environmental conditions. Recently, Streptococcus pneumoniae has been shown to co-ordinate the uptake of transforming DNA with fratricide via increased expression of the peptide pheromone responsible for competence induction. Here, we document that environmental stress-induced expression of the peptide pheromone competence-stimulating peptide (CSP) in the oral pathogen Streptococcus mutans. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL5911

6 Samples

Download data: MEV

(Submitter supplied) Polymicrobial biofilms are of large medical importance, but little is known about their physiology and the underlying interspecies interactions. Here we studied two human pathogens, the opportunistic fungus Candida albicans and the caries promoting bacterium Streptococcus mutans. Both species formed biofilms in monoculture, with C. albicans growing mainly in the virulence-associated hyphae form, and S. more...

Organism:

Candida albicans; Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL10540

16 Samples

Download data: TXT

(Submitter supplied) Multiple S. mutans genes that are subject to SloR control are likely contributors to virulence. The regulation of these genes in response to metal ion limitation vs. availability in the oral cavity can significantly facilitate the caries-forming process and hence continued analysis of these data can reveal novel candidate genes for the purposes of therapeutic intervention and/or prevention. Keywords: Genetic modification, dose response, metalloregulation

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL6395

10 Samples

Download data: CEL

(Submitter supplied) RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. more...

Organism:

Streptococcus oralis; Corynebacterium matruchotii; Streptococcus gordonii; Streptococcus sobrinus; Lacticaseibacillus casei; Streptococcus mutans UA159; Streptococcus sanguinis; Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

11 related Platforms

33 Samples

Download data: CSV, XLSX

(Submitter supplied) In the oral biofilm, the mitis streptococci are among the first group of organisms to colonize the tooth surface. Their proliferation is thought to be an important factor required for antagonizing the growth of cariogenic species such as Streptococcus mutans. In this study, we used a 3-species mixed culture to demonstrate that another ubiquitous early colonizing species, Veillonella parvula, could greatly impact the competitive outcome of a mixed culture of S. more...

Organism:

Streptococcus mutans; Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL4769

8 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of S. mutans cells comparing WT cells with lytS cells at early- and late-exponential growth phase.

Organism:

Streptococcus mutans; Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL15711

16 Samples

Download data: MEV

(Submitter supplied) To gain further insights into the molecular basis of the effects of oxygen on biofilm formation by S. mutans (Ahn and Burne, 2007), we used DNA microarrays to analyze gene expression profiles of cells cultured under aerobic or anaerobic conditions. Keywords: Oxygen, Biofilm, Caries, Microarray

Organism:

Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4340

8 Samples

Download data: MEV