GEO DataSets

(Submitter supplied) The integrity of the bacterial cell envelope is essential to sustain life by countering the high turgor pressure of the cell and providing a barrier against chemical insults. In Bacillus subtilis, synthesis of both peptidoglycan and wall teichoic acids requires a common C55 lipid carrier, undecaprenyl-pyrophosphate (UPP), to ferry precursors across the cytoplasmic membrane. The synthesis and recycling of UPP requires a phosphatase to generate the monophosphate form Und-P, which is the substrate for peptidoglycan and wall teichoic acid synthases. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

4 Samples

Download data: GPR

(Submitter supplied) To define the ECF sigma sigV - regulated genes during log growth phase in LB media under induction conditions for sigV The seven extracytoplasmic function (ECF) sigma (σ) factors of Bacillus subtilis are broadly implicated in resistance to antibiotics and other cell envelope stressors mediated, in part, by regulation of cell envelope synthesis and modification enzymes. We here define the regulon of σV as including at least 20 operons many of which are also regulated by σM, σX, or σW. more...

Organism:

Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL7420

12 Samples

Download data: GPR

(Submitter supplied) Abstract of associated manuscript: The Bacillus subtilis extracytoplasmic function (ECF) sigma(M) factor is activated by cell envelope stress elicited by antibiotics, and by acid, heat, ethanol and superoxide stresses. Here, we have used several complementary approaches to identify genes controlled by sigma(M). In many cases, expression is only partially dependent on sigma(M) because of both overlapping promoter recognition with other ECF sigma factors and the presence of additional promoter elements. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

14 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platforms:

GPL7420 GPL13726

15 Samples

Download data: GPR

(Submitter supplied) Transcriptional response of Bacillus subtilis to ramoplanin in wild-type CU1065.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL13726

12 Samples

Download data: GPR

(Submitter supplied) Transcriptional response of Bacillus subtilis to moenomycin in wild-type 168.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

3 Samples

Download data: GPR

(Submitter supplied) Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes.

Organism:

Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL13168

12 Samples

Download data: PAIR, TXT

(Submitter supplied) Identification of the specific PhoP binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the PhoPR regulon in Bacillus subtilis and its role in adaptation to phosphate-limiting conditions and cell wall metabolism, as well as implying a role in several other cellular processes.

Organism:

Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL13168

12 Samples

Download data: PAIR, TXT

(Submitter supplied) Transcriptional profile of the uppS-RBS (A to C) mutant during log growth phase in LB medium.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

4 Samples

Download data: GPR

(Submitter supplied) Genome-wide screens have discovered a large set of essential genes in the human pathogen Streptococcus pneumoniae. However, the function of many essential genes is still unknown, hampering vaccine and drug development programs. Based on results from transposon-sequencing (Tn-Seq), we refined the list of essential genes in S. pneumoniae serotype 2 strain D39. Next, we created a knockdown library targeting all 391 potentially essential genes using CRISPR interference (CRISPRi). more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18183

6 Samples

Download data: TXT

(Submitter supplied) Investigation of the whole genome expression level changes in phosphate limited Bacillus subtilis wild-type and delta-phoPR cells Investigation of the whole genome expression level changes of wild-type and delta-phoPR Bacills subtilis cells comparing high and low phosphate medium

Organism:

Bacillus subtilis subsp. subtilis str. 168; Bacillus subtilis

Type:

Expression profiling by genome tiling array

Platform:

GPL8486

18 Samples

Download data: PAIR

(Submitter supplied) Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase. Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL6031

4 Samples

Download data: TXT

(Submitter supplied) Transcriptional profile of the rocG gudB double null mutant during log growth phase in LB medium.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

2 Samples

Download data: GPR

(Submitter supplied) Effect on gene transcript levels upon up- or down-regulation of two component system WalRK (Wal-ON and Wal-OFF)

Organism:

Clostridioides difficile

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32143

21 Samples

Download data: CSV

(Submitter supplied) Synchronizing production of antibacterial compounds and integration of DNA released by dead cells, competence is one of the most efficient bacterial evolutionary and adaptative strategies. In most streptococci, this tactic is orchestrated by the ComRS system, a pheromone communication device providing competence bimodal initiation and sharp time window of activation. Understanding how this developmental process integrates multiple inputs to fine-tune the adequate response is a long-standing question. more...

Organism:

Streptococcus salivarius

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32298

3 Samples

Download data: XLSX

(Submitter supplied) We report the global changes in the gene expression profile of Caulobacter crescentus in the absence of Hfq.

Organism:

Caulobacter vibrioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21015

6 Samples

Download data: TXT

(Submitter supplied) Essential gene products underpin the core reactions required for cell viability, but are poorly studied in vivo due to a shortage of appropriate technologies. Using CRISPR interference, we created knockdowns of every essential gene in Bacillus subtilis and probed their phenotypes. Our high-confidence essential gene network, established using chemical genomics, showed extensive interconnections among distantly related processes and identified the modes of action for uncharacterized antibiotics. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL18561

8 Samples

Download data: WIG

(Submitter supplied) To obtain global cellular response of Bacillus subtilis WT W168 against the intrinsically produced antimicrobial peptide YydF, we performed RNA-seq experiments. For this, we synthesized YydF, extrinsically added 0.5µM to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition.

Organism:

Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27769

6 Samples

Download data: CSV, GFF, TXT