GEO DataSets

(Submitter supplied) Age-related frailty may in part be due to a decreased competency in skeletal muscle regeneration. The role of the closely related TGFbeta amily molecules myostatin and GDF11 in regeneration is unclear. The commercially available antibody which in a prior report was used to demonstrate an age-related decrease in GDF11 was found to detect both GDF11 and myostatin, and with this reagent it appears that the combination of GDF11 and myostatin increases with age in serum. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

56 Samples

Download data: CEL

(Submitter supplied) In this study, we investigated signaling pathways in Skeletal muscle precursors that are altered with aging and age-related deficits in muscle regenerative potential. We performed fluorescence activated cell sorting (FACS) to obtain highly purified skeletal muscle satellite cells from young, middle-aged and old mice. Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting that young blood contains humoral "rejuvenating" factors that can restore regenerative function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4892

Platform:

GPL1261

14 Samples

Download data: CEL

Analysis of FACS-sorted CD45-Ter119-Sca-1-CD29+Cxcr4+ skeletal muscle precursor (SMP) cells from young, middle-aged, and old C57BL/6 males. Results provide insight into the molecular mechanisms underlying age-related skeletal muscle and stem cell dysfunction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age sets

Platform:

GPL1261

Series:

GSE50821

14 Samples

Download data: CEL

(Submitter supplied) Here we used RNA-Seq to unravel the gene expression patterns and related signaling pathways that might be responsible for the observed changes in the lipidome upon GDF11 treatment of hepatocellular carcinoma cells (HCC), HepG2. RNA-seq analysis was performed after 24h of GDF11 (100ng/ml) treatment to investigate its involvement in lipid metabolism. Genes exhibiting absolute fold-change values >2 and p-values <0.05 were considered differentially expressed between contrasts and statistical differences in gene expression were assessed by the ANOVA test. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15520

6 Samples

Download data: TXT

(Submitter supplied) we identified novel age-related microRNAs through comparative analysis of miRNA expression profiles in young and old myoblasts using next generation sequencing (NGS)

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11002

8 Samples

Download data: TXT

(Submitter supplied) Growth differentiation factor 11 (GDF11), is one of the members of transforming growth factor β (TGFβ) superfamily. We set out to unequivocally reveal the effect of endogenous GDF11 on biological process by adopting CRISPR/Cas9 gene knockout strategy to specifically delete GDF11 gene in Neuro-2a cells. We analyze mRNA profiles of differentially genes in wild type (WT) and GDF11 knockout (GDF11-/-) Neuro-2a cells by using RNA-seq technology. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: BW

(Submitter supplied) The objective of this study was to identify transcriptional changes differentially regulated by GDF11 stimulation compared to TGFB1 Microarrays were used to capture the differential transcriptional response to acute GDF11 stimulation compared to acute TGFB1 stimulation

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

12 Samples

Download data: TXT

(Submitter supplied) GDF11 treatment leads to bone loss in mice and strongly stimulates RANKL-induced osteoclastogenesis of bone marrow–derived macrophages (BMMs) in vitro. We used microarrays to identified distinct classes of up-regulated genes in BMMs after GDF11 treatment.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL, CHP