GEO DataSets

(Submitter supplied) Plasmacytoid dendritic cells (pDCs) are key components of the innate immune response that are capable of synthesizing and rapidly releasing vast amounts of type I interferons (IFNs), particularly IFN-alpha. Here we investigated whether pDCs, often regarded as a mere source of IFN, discriminate between various functionally discrete stimuli and to what extent this reflects differences in pDC responses other than IFN-alpha release. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7088

95 Samples

Download data: TXT

(Submitter supplied) The goal is to study the difference in transcriptomic profile (RNA seq) of pDCs from IRF8WT and IRF8R294C mice.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: TXT

(Submitter supplied) Plasmacytoid dendritic cells (pDCs) represent a rare innate immune subset uniquely endowed with the capacity to produce substantial amounts of type-I interferons (IFN-I). This function of pDCs is critical for effective antiviral defenses and has been implicated in autoimmunity. While IFN-I and select cytokines have been recognized as pDC secreted products, a comprehensive agnostic profiling of the pDC secretome in response to a physiologic stimulus has not been reported. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

3 Samples

Download data: CSV, H5

(Submitter supplied) To evaluate gene expression profiles on different dendritic cell subsets isolated from spleens of mice We used whole genome microarrays to identify genes specific to pDCs and not other DC subsets in the absence of disease

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

3 Samples

Download data: CEL

(Submitter supplied) The steady-state gene expression of pDCs of two different mouse strains was analyzed. Splenic plasmacytoid dendritic cells (pDCs) of Siglec-H knock-out mice on C57Bl& background and the Siglec-H expression littermates (+/+) were enriched and sorted by FACS before generating RNA, followed by cDNA generation and sequencing.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

11 Samples

Download data: TXT

(Submitter supplied) Over 2600 genes were differentially expressed in pDCs exposed to influenza A compared with the controls (no virus). Multiple functional group clusters of genes were impacted, including those involving antiviral responses and also metabolism, such as the glycolysis, oxidative phosphorylation, glucose metabolic process, and positive regulation of macromolecule biosynthesis clusters.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS6063

Platform:

GPL10558

10 Samples

Download data: TXT

Analysis of primary plasmacytoid dendritic cells (pDC) exposed to influenza A for 8 hours ex vivo. pDCs are vital to antiviral defense, directing immune responses via secretion of interferon-alpha. Results provide insight into the regulation of the response of pDC to viral pathogens.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 individual, 2 infection sets

Platform:

GPL10558

Series:

GSE68849

10 Samples

Download data

(Submitter supplied) Plasmacytoid dendritic cells (pDCs) can rapidly produce interferons and other soluble factors in response to extracellular viruses or virus mimics such as CpG-containing DNA. pDCs can also recognize live cells infected with certain RNA viruses, but the relevance and functional consequences of such recognition remain unclear. We studied the response of primary DCs to the prototypical persistent DNA virus, the human cytomegalovirus (CMV). more...

Organism:

blank sample; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18573 GPL29107

96 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

240 Samples

Download data

(Submitter supplied) An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

120 Samples

Download data: TXT

(Submitter supplied) An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

120 Samples

Download data: TXT

(Submitter supplied) Blood plasmacytoid dendritic cells (pDCs) purified from anonymous donors were stimulated ex vivo with rhinovirus-16 (RV) in the presence or absence of eosinophil supernatants. Purified pDCs from 8 individuals with moderate-severe asthma, treated or not treated with anti-IL-5/5Rα therapy, were cultured with or without RV. Transcriptome analysis revealed global repression of pDC IFN response patterns by eosinophils, most notably in basal expression of interferon stimulated genes (ISGs). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

42 Samples

Download data: CSV

(Submitter supplied) IRF7 plays a critical role in the production and amplification of the antiviral type I and III interferon response. Autosomal recessive IRF7-deficiency resulted in life-threatening influenza disease in a 3-year-old child. We studied the impact of IRF7-deficiency in non-hematopoietic tissues (fibroblasts and lung epithelial cells) as well in hematopoietic cells (peripheral blood mononuclear cells (PBMCs)). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5626

Platform:

GPL10558

18 Samples

Download data: TXT

Analysis of PBMCs isolated from an IRF7-deficient patient and infected with influenza A/CA/4/2009 for up to 16 hrs. Transcription factor IRF7 amplifies type I and III interferon response to viruses. Results provide insight into the role of IRF7 in antiviral immunity.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 6 individual, 2 infection, 3 time sets

Platform:

GPL10558

Series:

GSE66486

18 Samples

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(Submitter supplied) HIV-1 and HIV-2 can both infect humans, but HIV-2 causes a slow progressing disease and is well controlled by the immune system for prolonged period of times. Here we tested acute response aainst HIV-1 and HIV-2 exposure in peripheral blood mononuclear cells (PBMC) from three indepednent donors.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

24 Samples

Download data: CEL

(Submitter supplied) Plasmacytoid dendritic cells (pDC) are the main source of type I interferon (IFN) during viral infections. Their other functions are debated, due to a lack of tools to identify and target them in vivo without affecting pDC-like cells and transitional DC (tDC), which harbor overlapping phenotypes and transcriptomes but a higher efficacy for T cell activation. To overcome this bottleneck, we designed, generated and validated a pDC-Tomato reporter mouse. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30172

14 Samples

Download data: CSV, TXT

(Submitter supplied) To understand how the transcriptome of pDCs is impacted by HIV-1 infection and exogenous stimulation, we sorted pDCs from people with HIV-1 (PWH) on antiretroviral treatment before and after toll-like receptor 9 (TLR9) agonist treatment, as well as from healthy controls, and performed single-cell (sc)-RNA sequencing. We generated a transcriptomic atlas of over 112,000 pDCs. Our cluster analysis revealed an inducible cytotoxic-like pDC cluster characterized by high expression of both antiviral and cytotoxic genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

12 Samples

Download data: TAR

(Submitter supplied) Humans show remarkable variation in susceptibility to infectious diseases as well as chronic inflammatory and autoimmune disorders. This heterogeneity arises partially from variation in the immune response, which is responsible for preventing and controlling infection. To better understand the major factors driving antiviral immune response differences, we used single-cell RNA-sequencing to measure the effects of genetic ancestry and cis-regulatory variation on the transcriptional response to influenza infection in various immune cell types in 90 European and African American individuals. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

292 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) Purpose: to analyze gene expression changes of iMACs encountering S. pneumoniae. Methods: SMART-seq2 amplified polyA RNA from sorted iMACs in the co-cultures. Results: the transcriptome of iMACs changed after encountering S. pneumoniae. Conclusions: the gene expression profiles of S. pneumoniae stimulated iMACs indicated iMACs undergoing inflammatory response and the immune response was distinct with iMACs encountering other pathogens such as HCV or SARS-CoV-2.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

4 Samples

Download data: TXT

(Submitter supplied) Purpose: to depict the single-cell map and reveal the heterogeneity of hPSC derived macrophages Methods: Differentiation day 14 iMacs were dissociated to obtain a single-cell suspension. scRNA-seq and scATAC-seq library preparation and sequencing were done by CapitalBio Technology (https://www.capitalbiotech.com/). scRNA-seq, the cell suspension was immediately loaded onto the Chromium Single Cell Controller (10× Genomics) for droplet formation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

2 Samples

Download data: BED, MTX, TBI, TSV