GEO DataSets

(Submitter supplied) Transcriptional profiling of yeast Saccharomyces serevisiae cells exposed to stress (30 min) compared to no stress conditions (YPD) Two stress condition experiments, YP20 and YPS, each one compared to no-stress conditions (YPD).

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL9825

4 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of yeast Saccharomyces serevisiae cells exposed to stress (30 min) compared to no stress conditions (YPD) in a hot1delta mutant Two stress condition experiments, YP20 and YPS, each one compared to no-stress conditions (YPD) in a hot1delta mutant

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL9825

4 Samples

Download data: TXT

(Submitter supplied) These three replicates were analyzed in "Genomewide identification of Sko1 target promoters reveals a regulatory network that operates in response to osmotic stress in Saccharomyces cerevisiae. ", by Proft M, Gibbons FD, Copeland M, Roth FP, Struhl K; published in Eukaryot Cell. 2005 Aug;4(8):1343-52. A new analysis algorithm for Chip-chip data ('Chipper') is described in Genome Biology. Manuscript entitled "Chipper: discovering transcription-factor targets from chromatin immunoprecipitation microarrays using variance stabilization." by FD Gibbons, M Proft, K Struhl, and FP Roth. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL1689

3 Samples

Download data

(Submitter supplied) The experiment was designed to study the transcriptional profiling of Candida albicans wild-type CAI-4 strains and corresponding Histatin 5 treated strains. Since Histatin 5 will kill Candida albicans, the focus will be on how many and which genes are significantly affected with the treatment of Histatin 5. Currently, we only conducted the microarray experiments at one concentration of Histatin 5 and one time point of treatment. more...

Organism:

Candida albicans; Candida albicans SC5314

Type:

Expression profiling by array

Platform:

GPL4920

4 Samples

Download data: GPR

(Submitter supplied) When challenged with osmotic shock, S. cerevisiae induces hundreds of genes, despite a global reduction in transcriptional capacity. The mechanisms that regulate this rapid reallocation of transcriptional resources are not known. Here we show that redistribution of RNA Pol II upon stress requires the stress-responsive MAP kinase Hog1. We find that Hog1 and RNA Pol II co-localize to open reading frames that bypass global transcriptional repression, and that these targets are specified by two osmotic stress-responsive transcription factors. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9377

18 Samples

Download data: TXT

(Submitter supplied) We analyzed the effect of the elimination of the cap-binding protein Cbc1 on transcription rate and mRNA amount during the response to osmotic stress using the Genomic Run-on assay

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array; Other

Platform:

GPL13620

20 Samples

Download data: TXT

(Submitter supplied) Yeast cells can be affected during their growth to several stress conditions. One of the most known and characterised is the osmotic stress and most of the studies about osmotic sterss response in yeast have been focused on salt or sorbitol stress. However, during yeast growth in industrially relevant processes (for instance throughout alcoholic fermentation on the must to produce alcoholic beverages) the osmotic stress is mainly due to the high sugar(in particular glucose) concentration (200-250 g/L).

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1914

5 Samples

Download data: GPR, TXT

(Submitter supplied) We examine how SUMO post-translational modification of the yeast transcription factor Sko1 affects its binding site selection and affinity. Chromatin immunoprecipitation followed by next-generation sequencing was performed in strains expressing wild-type Sko1 (Sko1-WT) or Sko1 that harbors an Arg-to-Lys mutation at Lys 567 (Sko1-MT), that impairs its sumoylation. We find that, compared with Sko1-WT, SUMO-deficient Sko1 binds numerous additional sites that are near promoters of non-Sko1 target genes. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17342

12 Samples

Download data: TXT

(Submitter supplied) Complex biological processes are often regulated, at least in part, by the binding of transcription factors to their targets. Recently, considerable effort has been made to analyze the binding of relevant factors to the suite of targets they regulate, thereby generating a regulatory circuit map. However, for most studies the dynamics of binding have not been analyzed and thus the temporal order of events and mechanisms by which this occur are poorly understood. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL3541

60 Samples

Download data: PAIR

(Submitter supplied) In this study, we aim to understand how the fates of stress-activated mRNAs are determined under stress conditions by isolating individual osmostress-activated mRNA species, quantitating the proteins associated in vivo with each of them, and analyzing how deletion of these proteins impact on the expression of stress-activated genes. By comparison with the proteome associated with individual not stress-related mRNA species, we show specific proteins to be preferentially binding to osmostress-activated mRNAs, notably members of the cytoplasmic Pat1 / Lsm1 – 7 complex. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL19756

18 Samples

Download data: BEDGRAPH

(Submitter supplied) The Crabtree effect, in which fermentative metabolism is preferred at the expense of respiration, is a hallmark of budding yeast’s glucose response and a model for the Warburg effect in human tumors. While the glucose-responsive transcriptional repressors Mig1p and Mig2p play well-characterized roles in the Crabtree effect, little function for the related Mig3p transcription factor has been uncovered despite numerous investigations of laboratory yeast strains. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL13254

19 Samples

Download data: FTR, TXT

(Submitter supplied) Recent single-cell studies have revealed that yeast stress response involves multiple transcription factors that are temporally activated in pulses. However, it remains largely unclear whether and how these dynamic transcription factors temporally interact to regulate stress survival. Here we show that budding yeast cells can exploit the temporal relationship between paralogous general stress regulators, Msn2 and Msn4, during stress response. more...

Organism:

Saccharomyces cerevisiae; Nakaseomyces glabratus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29410 GPL26171

36 Samples

Download data: CSV

(Submitter supplied) Virtually all living organisms are challenged by changes in their environment, such as sudden increases in osmolarity. To survive, cells have developed stress-responsive mechanisms that tune functions such as metabolism and gene expression. The response of Saccharomyces cerevisiae to osmostress includes a massive reprogramming of gene expression. Identifying the inherent features of stress-responsive genes is of significant interest for understanding the basic principles underlying the rewiring of gene expression upon stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17342

18 Samples

Download data: TXT