GEO DataSets

(Submitter supplied) Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) which has been shown to increase the susceptibility of various bacteria to antimicrobials and demonstrated to have broad antimicrobial activity. This study describes transcriptome alterations in S. aureus strain COL grown with diclofenac and characterizes the effects of this NSAID on antibiotic susceptibility in laboratory, clinical and diclofenac reduced-susceptibility (DcRS) mutant S. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus COL

Type:

Expression profiling by array

Platform:

GPL5879

4 Samples

Download data: TXT

(Submitter supplied) Genome-wide transcriptional profiling studies of the growth of bacteria with antimicrobial agents often reveals aspects of the drug-specific protective bacterial response. Fusidic acid is a steroid antimicrobial that inhibits protein synthesis by interfering with the release of elongation factor G (EF-G) after it has functioned in the translocation step. Two hundred and seventy two genes were both up- and down-regulated in a fusidic acid-susceptible strain of Staphylococcus aureus following challenge with 2 mg/L-1 fusidic acid for 15 min. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL7072

4 Samples

Download data: TAV

(Submitter supplied) The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids that is produced by Bacillus sp. strain HIL Y-85,54728. Lantibiotics are antibiotics containing nonproteinogenic amino acids like lanthionine and/or 3-methyllanthionine. Mersacidin interferes with cell wall biosynthesis by targeting the peptidoglycan precursor lipid II and inhibits the growth of MRSA and other gram-positive bacteria. more...

Organism:

Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5421

2 Samples

Download data: GPR

(Submitter supplied) Telavancin is a novel semi-synthetic lipoglycopeptide derivative of vancomycin with a dual mode of action. This study sought to understand knowledge of mechanisms of decreased susceptibility in laboratory-derived mutant. There were multiple significant changes in the transcriptome of TlvDSMED1952 indicating an altered overall physiology of the strain, likely decreased virulence, and changes in cell wall and membrane properties, probably due to multiple genetic changes in the strain.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10591

3 Samples

Download data: TXT

(Submitter supplied) Understanding how pathogens respond to antimicrobial peptides, and how this compares to currently available antibiotics, is crucial to optimizing antibiotic therapy. Staphylococcus aureus has several known resistance mechanisms against human cationic antimicrobial peptides (CAMPs). We aim to determine how S. aureus responds to sheep and frog CAMPs, and whether this response is associated with resistance. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL3931

12 Samples

Download data: TXT

(Submitter supplied) To analyze function of three Two-component systems SAOUHSC_00184-185, SAOUHSC_01313-1314, and SAOUHSC_01980-1981, we have employed whole genome microarray expression profiling to identify genes transcription changed in respective mutant strains. Target TCSs were replaced by ermB gene.

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325

Type:

Expression profiling by array

Platform:

GPL17776

4 Samples

Download data: TXT

(Submitter supplied) In the present study we analyzed the response of S. aureus to mupirocin, the drug of choice for nasal decolonization of S. aureus. Mupirocin selectively inhibits the bacterial isoleucyl-tRNA synthetase (IleRSs) leading to the accumulation of uncharged isoleucyl-tRNA and hence (p)ppGpp. The latter is a signal for the induction of the stringent response, an important global transcriptional and translational control mechanism that allows bacteria to adapt to nutritional deprivation. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL13937

4 Samples

Download data: TXT

(Submitter supplied) Daptomycin is a lipopeptide antibiotic that has recently been approved for treatment of Gram-positive bacterial infections. The mode of action of daptomycin is not yet entirely clear. To further understand the mechanism transcriptomic analysis of changes in gene expression in daptomycin-treated Staphylococcus aureus was carried out. The expression profile indicated that cell wall stress stimulon member genes (B. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL6059

6 Samples

Download data: TXT

(Submitter supplied) Investigation of baseline transcription activity of two different clinical isolates of Staphylococcus aureus with two different susceptibility levels to the antibiotics Vancomycin and Daptomycin.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15888

2 Samples

Download data: TXT

(Submitter supplied) Staphylococcus aureus is a notorious bacterial pathogen that causes a broad range of human diseases, and isolates that are resistant to several antibiotic classes including last resort antibiotics like vancomycin and daptomycin complicate the situation. We characterized S. aureus VC40, a strain that shows full resistance to vancomycin (MIC of 64 µg/ml) and daptomycin (MIC of 4 µg/ml) as well as a decreased susceptibility to further cell wall active agents. more...

Organism:

Staphylococcus aureus subsp. aureus VC40; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10537

6 Samples

Download data: TXT

(Submitter supplied) A triclosan-ciprofloxacin cross-resistant mutant strain of Staphylococcus aureus displays an alteration in the expression of several cell membrane structural and functional genes. Triclosan is an antimicrobial agent found in many consumer products. Several studies have demonstrated that triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Studies have also demonstrated that decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Dataset:

GDS3136

Platform:

GPL1339

12 Samples

Download data: CEL

Analysis of Staphylococcus aureus JJ5 mutant strain treated with triclosan. JJ5 is a triclosan-ciprofloxacin cross-resistant strain. Triclosan resistance correlates with ciprofloxacin resistance in several bacteria. Results provide insight into the molecular basis of triclosan resistance in JJ5.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array, count, 2 agent, 2 strain sets

Platform:

GPL1339

Series:

GSE5799

12 Samples

Download data: CEL

(Submitter supplied) Complete reconstitution of the vancomycin-intermediate Staphylococcus aureus (VISA) phenotype of Mu50 was achieved by sequentially introducing mutations into five genes of a vancomycin-susceptible S. aureus (VSSA) strain ∆IP. Introduction of mutation Ser329Leu into vraS encoding the sensor histidine kinase of vraSR two-component regulatory (TCR) system and another mutation Glu146Lys into msrR, encoding putative methionine sulfoxide reductase regulator, raised vancomycin resistance to the level of heterogeneously vancomycin-intermediate S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL16176

19 Samples

Download data: PAIR

(Submitter supplied) S. aureus and MRSA are susceptible to TTO and are therefore targeted in nascent TTO clinical trails. We now report the alterations in the transcriptome of a S. aureus exposed to a growth inhibitory concentration of TTO. These efforts have uncovered additional mechanisms by which this membrane active antimicrobial substance inhibits bacterial growth.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5879

4 Samples

Download data: TXT

(Submitter supplied) Ethanol is utilized widely in medicine for both its antiseptic and disinfectant capabilities. In an effort to understand the effects of ethanol on a very common human pathogen, we have determined the ethanol-stimulon of two well characterized unrelated clinical S. aureus methicillin resistant strains LP9 and MM66 with ethanol minimum inhibitory concentrations (MIC) of 10 and 7% respectively. Using S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5879

6 Samples

Download data: TXT

(Submitter supplied) The GntR-like protein NorG has been shown to affect Staphylococcus aureus genes involved in the resistance to quinolones and beta-lactams such as those encoding the NorB and AbcA transporters. To identify the target genes regulated by NorG, we carried out transcriptional profiling assays using S. aureus RN6390 and its isogenic norG::cat mutant. Our data showed that NorG positively affected the transcription of global regulators mgrA, arlS, and sarZ. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

6 Samples

Download data: CEL, CHP

(Submitter supplied) Overnight cultures of wild type COL or the bshA mutant were back-diluted to an O.D.650 of 0.1 in 30 mL of TSB. The cells were grown at 37C with shaking in a 300mL flask until they reached an O.D.650 of 1.1. The cells were then equally separated into 50mL Falcon tubes, so that each tube had 10mL of culture. One tube for each strain was induced with 1 mM diamide and one with 10 mM H2O2, which we did not send for analysis. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL11137

12 Samples

Download data: TXT

(Submitter supplied) S. aureus possesses 16 two-component systems (TCS), two of which (GraRS & NsaRS) belong to the intramembrane-sensing histidine kinase (IM-HK) family, conserved within the firmicutes. NsaRS has recently been documented as being important for nisin-resistance in S. aureus. In this study we present a detailed characterization of NsaRS and reveal that, as with other IM-HK TCS, it responds to disruptions in cell-wall stability. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5879

4 Samples

Download data: GPR

(Submitter supplied) Daptomycin is an extensively used anti-staphylococcal agent due to the rise in methicillin-resistant Staphylococcus aureus. However, both laboratory-derived and clinical decreased susceptibility isolates have been described, but the mechanism(s) of resistance is poorly understood. To further understand daptomycin resistance, comparative genome sequencing, transcriptomics, ultrastructure ,and cell envelope studies were carried out on two relatively higher level (4 and 8 ug/ml-1) laboratory-derived daptomycin-resistant strains (strains CB1541 and CB1540 respectively) compared to their methicillin-resistant parent strain (CB1118;MW2). more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5881

6 Samples

Download data: TXT

(Submitter supplied) S. aureus response to exogenous fatty acid (oleic acid) Gene expression profiles were generated by microarray analysis of S. aureus cells grown in media without or with oleic aicd

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus RN4220

Type:

Expression profiling by array

Platform:

GPL1339

6 Samples

Download data: CEL, CHP