GEO DataSets

(Submitter supplied) The insulin-like signaling (ILS) pathway regulates metabolism and is known to modulate adult lifespan in C. elegans. Altered stress responses and resistance to a wide range of stressors are also associated with changes in ILS and contribute to enhanced longevity. The transcription factors DAF-16 and HSF-1 are key effectors of the longevity phenotype. We demonstrate that increased intrinsic thermotolerance, due to lower ILS, is not dependent on stress induced HSF-1 transcriptional responses but instead requires active protein translation. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9458

72 Samples

Download data: GPR

(Submitter supplied) The goal of this experiment was to identify transcripts regulated non-autonomously by age-1 activity in neurons or intestine. Comparisons of gene expression were made between age-1(mg44) null adults and CY251 (age-1(mg44); bvIs2) and CY262 (age-1(mg44); bvIs1); all were relative to wildtype (N2). The integrated transgenes, bvIs2 and bvIs1, carry the age-1 cDNA expressed from the neuronal ric-19 promoter (bvIs2) or intestinal ges-1 promoter (bvIs1). more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL2875

8 Samples

Download data: TXT

(Submitter supplied) In order to understand the complexity of gene regulation downstream of IIS, we did RNA-seq in mixed culture in wild-type, daf-2(e1370), daf-16(mgDf50);daf-2(e1370) and daf-2(e1370);daf-12(m20 and correlated it with ChIP-seq data

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13776

6 Samples

Download data: TXT

(Submitter supplied) In order to understand the complexity of gene regulation downstream of IIS, we used anti-DAF-16 antibody, we report the first genome-wide ChIP-sequencing study of endogenous DAF-16 recruitment in daf-2(e1370). We also report the average bin-wise normalized read count of ZFP-1 and DAF-16 on DAF-16 summits

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13776

8 Samples

Download data: TXT

(Submitter supplied) The gene expression profile of WT (N2) C. elegans were compared to that of ets-4(ok165) and ets-4(uz1) mutant animals

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3747

Platform:

GPL7272

6 Samples

Download data: TXT

Analysis of stage L4 larvae with two distinct deletion alleles of ets-4: ets-4(ok165) and ets-4(uz1). Adults with ets-4 mutations have a significant extension of mean life span. Results provide insight into the transcriptional control of aging effectors by the ETS-4 transcription factor.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, log2 ratio, 2 genotype/variation sets

Platform:

GPL7272

Series:

GSE21851

6 Samples

Download data: TXT

(Submitter supplied) Analysis of gene expression in long-lived daf-2 mutants for the insulin/IGF-1 receptor. daf-2(e1370) and daf-2(m577) alleles each examined as single and as double mutations with the daf-16(df50) allele. DAF-2 regulates DAF-16, a transcription factor. Results provide insight into longevity. Keywords: other

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS770

Platform:

GPL200

20 Samples

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Analysis of gene expression in long-lived daf-2 mutants for the insulin/IGF-1 receptor. daf-2(e1370) and daf-2(m577) alleles each examined as single and as double mutations with the daf-16(df50) allele. DAF-2 regulates DAF-16, a transcription factor. Results provide insight into longevity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 4 genotype/variation sets

Platform:

GPL200

Series:

GSE1762

20 Samples

Download data

(Submitter supplied) Synchronized C. elegans cultures of three geontypes -- wildype N2, daf-2(e1370) and sma-6(wk7) -- were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated at 25C for 4 or 24 hours before harvesting. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

4 related Platforms

26 Samples

Download data: TXT

(Submitter supplied) Transcriptome analysis of a population of wild type animals and lsm-1 mutants at L3 stage

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13776

2 Samples

Download data: XLS

(Submitter supplied) Purpose: We used RNA-Seq to compare the gene expression profiles in two long-lived C. elegans strains: (1) animals with a loss-of-function mutation in the hsb-1 gene, and (2) animals overexpressing hsf-1 under its endogenous promoter. Previous studies indicated that life span extension in both these strains is hsf-1-dependent. Methods: mRNA-Seq profiles for three biological replicates each of day 1 adult wild-type (N2 strain), hsb-1 mutant and hsf-1 overexpression strains were generated using 50 bp single-end sequencing on an Illumina HiSeq 2500 platform. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

9 Samples

Download data: TXT

(Submitter supplied) Analysis of genes differentially expressed between daf-2(e1368) and daf-2(e1368);pmk-1(km25) and between daf-2(e1368) and daf-2(e1368);daf-16(mgDf47). These studies identified genes upregulated by wild-type PMK-1 and wild-type DAF-16. Keywords: genetic modification

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3253

Platform:

GPL200

9 Samples

Download data: CEL

(Submitter supplied) Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: Time course, response to pathogen infection

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3252

Platform:

GPL200

18 Samples

Download data: CEL

Analysis of daf-2(e1368) loss-of-function mutant and daf-2(e1368);pmk-1(km25) and daf-2(e1368);daf-16(mgDf47) double mutants. PMK-1 and DAF-16 confer enhanced pathogen resistance on daf-2 mutants relative to wild-type. Results suggest DAF-16 and PMK-1 upregulate distinct genes to promote immunity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 3 genotype/variation sets

Platform:

GPL200

Series:

GSE5801

9 Samples

Download data: CEL

Analysis of strain fer-15(b26);fem-1(hc17) young adults exposed for up to 8 hrs to pathogenic Pseudomonas aeruginosa strain PA14, less pathogenic PA14 mutant gacA, and E. coli strain OP50 (normal food source). Results provide insight into the molecular basis of the response to a bacterial pathogen.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 3 infection, 2 time sets

Platform:

GPL200

Series:

GSE5793

18 Samples

Download data: CEL

(Submitter supplied) Heat shock factor 1 (HSF-1) and forkhead box O (FOXO) are key transcription factors that protect cells from various stresses. InCaenorhabditis elegans, HSF-1 and FOXO together promote a long life span when insulin/IGF-1 signaling (IIS) is reduced. However, it remains poorly understood how HSF-1 and FOXO cooperate to confer IIS-mediated longevity. Here, we show that prefoldin 6 (PFD-6), a component of the molecular chaperone prefoldin-like complex, relays longevity response from HSF-1 to FOXO under reduced IIS. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

12 Samples

Download data: TXT

(Submitter supplied) To identify DAF-16-dependent transcriptional alterations that occur in a long-lived C. elegans strain, we used cDNA microarrays and genomic analysis to identify putative direct and indirect DAF-16 transcriptional target genes. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL2653

4 Samples

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(Submitter supplied) PMK-1 is involved in the heat stress response of C. elegans, translocates to the nucleus upon heat exposure and influences the expression of chaperone genes, proteasomal subunits and protein-biosynthesis related genes.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

2 Samples

Download data: TXT

(Submitter supplied) Insulin and insulin-like growth factor signalling regulates a broad spectrum of growth and metabolic responses to a variety of internal and environmental stimuli. Such responses can be tailored so that changes in insulin signalling result in distinct physiological responses to different stimuli. For example, the inhibition of insulin-like signalling is key in the responses of the nematode C. elegans to both osmotic stress and starvation, but these two stresses result in responses that are both physiologically and molecularly distinct. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19757

22 Samples

Download data: TXT

(Submitter supplied) Abstract: C. elegans SIR-2.1, a member of the Sir-2 family of NAD(+)-dependent protein deacetylases, has been shown to regulate nematode aging via the insulin/IGF pathway transcription factor daf-16. Treatment of C. elegans with the small molecule resveratrol, however, extends life span in a manner fully dependent upon sir-2.1, but independent of daf-16. Microarray analysis of worms treated with resveratrol demonstrates the transcriptional induction of a family of genes encoding prion-like glutamine/asparagine-rich proteins involved in endoplasmic reticulum (ER) stress response to unfolded proteins. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platforms:

GPL3390 GPL3391

12 Samples

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