GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

81 Samples

Download data: CEL

(Submitter supplied) To determine whether optic nerve head astrocytes, a key cellular component of glaucomatous neuropathy, exhibit differential gene expression in primary culture of astrocytes from African American donors with or without glaucoma, compared to astrocytes from Caucasian American donors with or without glaucoma. Keywords: Gene expression profile

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL571 GPL8300 GPL91

63 Samples

Download data: CEL

(Submitter supplied) Astrocytes from optic nerve head from donors with and without glaucoma Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1112

Platforms:

GPL8300 GPL91

15 Samples

Download data: CEL, EXP

Expression profiling of astrocytes taken from the optic nerve head (ONH) of patients with glaucoma. The ONH is the site of damage in glaucomatous optic neuropathy. Studies suggest that damage in glaucoma is mediated by reactive astrocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL8300

Series:

GSE2378

13 Samples

Download data: CEL, EXP

(Submitter supplied) Purpose: Marked extracellular matrix (ECM) remodeling occurs in the human optic nerve head in primary open angle glaucoma (POAG). The glial fibrillary acid protein (GFAP) negative lamina cribrosa cell may play an important role in this remodeling process. The authors report the first study of global and ECM-focused gene transcription differentials between GFAP-negative negative lamina cribrosa (LC) cells from normal and POAG human donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

4 Samples

Download data: CEL

(Submitter supplied) Gene expression in human optic nerve head (ONH) astrocytes exposed to either 60 mm Hg hydrostatic pressure (HP) or control ambient pressure (CP) was compared using Affymetrix GeneChip microarrays to identify HP-responsive genes. Primary ONH astrocytes from two male Caucasian donors (passage 4) were grown to 75% confluence and were exposed for 6, 24 or 48 h to control ambient pressure (CP6, CP24, CP48) or hydrostatic pressure (HP6, HP24, HP48), or harvested at the beginning of the pressure experiment (CP0). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS532

Platform:

GPL8300

44 Samples

Download data

Temporal analysis of how astrocytes in the optic nerve head (ONH) respond to changes in intraocular pressure (IOP). ONH astrocytes exposed to 60 mm Hg hydrostatic pressure for 6, 24, and 48 hours. Lends understanding to pathogenesis of glaucoma.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol, 4 time sets

Platform:

GPL8300

Series:

GSE758

44 Samples

Download data

(Submitter supplied) Primary open angle glaucoma (POAG) is a progressive optic neuropathy, which is a major cause of worldwide visual impairment and blindness. Pathological hallmarks of the glaucomatous optic nerve head include retinal ganglion cell axon loss and extracellular matrix (ECM) remodeling of the lamina cribrosa layer. TGF-beta is an important pro-fibrotic modulator of ECM metabolism, whose levels are elevated in human POAG lamina cribrosa tissue compared with non-glaucomatous controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1313

Platform:

GPL96

4 Samples

Download data

Analysis of glial fibrillary acidic acid (GFAP)-negative lamina cribrosa (LC) glial cell response to 10 ng/ml TGF-beta1 for 24 hours. This is a model for primary open-angle glaucoma (POAG) in which TGF-beta levels are elevated in human LC tissue.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL96

Series:

GSE2705

4 Samples

Download data

(Submitter supplied) The mechanical effect of raised intraocular pressure is a recognised stimulus for optic neuropathy in primary open angle glaucoma (POAG). Characteristic extra-cellular matrix (ECM) remodelling accompanies axonal damage in the lamina cribrosa (LC) of the optic nerve head in POAG. Glial cells in the lamina cribrosa may play a role in this process but the precise cellular responses to mechanical forces in this region are unknown. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

2 Samples

Download data

(Submitter supplied) Background: African Americans (AA) have increased burdens of cardiovascular disease and cancer compared to Caucasian Americans (CA). This study addresses the possibility that genetic differences affecting the biology of the vascular endothelium could be a factor contributing to this health disparity. Methods: From self-identified, healthy, 20-29 year old AA (n=21) and CA (n=17), we established cultures of blood outgrowth endothelial cells (BOEC) and applied microarray profiling. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

38 Samples

Download data: CEL

(Submitter supplied) The total RNA from control and POAG optic nerve lamina cribrosa region was isolated and subjected to analysis on U133A 2.0 affymetrix microarray chip. The results from these two analysis was compared to determine whether PAD II RNA level is altered. Purpose of the study. This study was done to determine which RNA transcripts undergo significant changes in the optic nerve between normal and cadaver primary open-angle glaucoma donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

2 Samples

Download data

(Submitter supplied) The incidence and mortality of colorectal cancer (CRC) is higher in African Americans (AAs) than in other ethnic groups in the U. S., but reasons for the disparities are unknown. We performed gene expression profiling and microsatellite instability (MSI) analysis of sporadic CRCs from AAs vs. European Americans (EAs) to assess the contribution to CRC disparities. We evaluated gene expression of 43 AA and 43 EA CRC tumors matched by stage and 40 normal colon tissues using the Agilent human whole genome 4x44K cDNA arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL1708 GPL4133

126 Samples

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(Submitter supplied) Glucocorticoids (GCs) are steroid hormones widely used as pharmaceutical interventions, which act mainly by regulating gene expression levels. A large fraction of patients (~30%), especially those of African descent, show a weak response to treatment. To interrogate the contribution of variable transcriptional response to inter-ethnic differences, we measured in vitro lymphocyte GC sensitivity (LGS) and transcriptome-wide response to GCs in peripheral blood mononuclear cells (PBMCs) from African-American and European-American healthy donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

48 Samples

Download data: TXT

(Submitter supplied) RNA-sequencing (RNA-seq) allows quantitative measurement of expression levels of genes and their transcripts. In this study, we sequenced complementary DNA fragments of cultured human B-cells and obtained 879 million 50-bp reads comprising 44 Gb of sequence. The results allowed us to study the gene expression profile of B-cells and to determine experimental parameters for sequencing-based expression studies. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9052

20 Samples

Download data: MAP, TXT

(Submitter supplied) We used microarrays to examine gene expression levels in individuals from different populations (cephgp, chb, jpt, yri). The chb and jpt populations are combined in the analysis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL201

388 Samples

Download data: CEL