GEO DataSets

(Submitter supplied) Expression profile of miRNAs during retinoic acid (RA) induced differentiation of mose embryonic stem cells Expression profile of mRNAs during retinoic acid (RA) amd miR-134 induced differentiation of mouse embryonic stem cells. Keywords: affymetrix custom array and illumina MouseRef-8

Organism:

Mus musculus

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL3576 GPL4865

30 Samples

Download data

(Submitter supplied) MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides in length that usually suppress the translation of target messenger RNAs (mRNAs) through partial complementarity to the 3¡¦ untranslated region (3¡¦ UTR) of protein-coding mRNAs in animals. However, there is increasing evidence that miRNAs can also reduce the steady-state levels of their target mRNAs in animals. In this investigation, both miRNA and mRNA profiles from the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF) were quantitatively determined. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) We have analyzed the transcript expression levels in Dicer knock-out embryonic stem (ES) cells 24 hours after transfection with either control siRNA agains Renilla luciferase or miRNA Mimics (Dharmacon) of mmu-miR-290 cluster members in order to identify primary targets of miR-290 cluster miRNAs. Keywords: Comparison of effect of two different transfections on transcriptome of Dicer-KO ES cells

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3430

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) We have analyzed the transcript expression levels in Dicer heterozygous and Dicer knock-out embryonic stem (ES) cells in order to identify which transcripts are regulated by RNAi pathway in mouse ES cells. Keywords: Cell type comparison of cell line with or without knock-out

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3404

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

Analysis of undifferentiated Dicer deficient embryonic stem (ES) cells transfected with the siRNA-like form of miRNAs of the miR-290 cluster. Loss of miRNA pathway components negatively affects ES cell differentiation. Results provide insight into the role of miR-290 in ES cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1261

Series:

GSE8503

6 Samples

Download data: CEL, CHP

Analysis of embryonic stem (ES) cells homozygous or heterozygous null for Dicer, an RNase III endonuclease required for processing microRNAs. Loss of microRNA pathway components negatively affects ES cell differentiation. Results provide insight into the role of microRNAs in ES cell development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE7141

6 Samples

Download data: CEL, CHP

(Submitter supplied) Genome-wide analysis of gene expression changes in murine embryonic stem cells (R1E cells) treated with Ultraviolet and adriamycin Both p53 and the Wnt signaling pathways play important roles in tumorigenesis and development. However, few studies, particularly on a genome-wide scale, have linked these two pathways. Here we show that p53 directly regulates the Wnt signaling pathway in murine embryonic stem cells (mESCs) using an integrated genome-wide approach. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) Both p53 and the Wnt signaling pathways play important roles in tumorigenesis and development. However, few studies, particularly on a genome-wide scale, have linked these two pathways. Here we show that p53 directly regulates the Wnt signaling pathway in murine embryonic stem cells (mESCs) using an integrated genome-wide approach. A chromatin-immunoprecipitation-based microarray assay (ChIP-chip) reveals that the Wnt signaling pathway is significantly over-represented in p53 bound genes. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by array

Platforms:

GPL4128 GPL4129

4 Samples

Download data: TXT

(Submitter supplied) To understand the specific mechanism by which Foxj3 and Zbtb18 control RA-induced neural directional differentiation of ESCs, total mRNAs of Foxj3-ES and Zbtb18-ES were extracted and used for RNA seq and transcriptome analyses. Compared with the control, 1331 genes were differentially expressed (P < 0.05) by twofold in Foxj3-ES (557 were underexpressed and 774 were overexpressed), and 1175 genes were differentially expressed (P < 0.05) by twofold in Zbtb18-ESCs (548 were underexpressed and 627 were overexpressed). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

9 Samples

Download data: XLS

(Submitter supplied) Dental pulp cells (DPCs) are known to be enriched in stem/progenitor cells but not well characterized yet. Small non-coding microRNAs (miRNAs) have been identified to control protein translation, mRNA stability and transcription, and have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In order to characterize dental pulp stem/progenitor cells and its mechanism of differentiation, we herein sorted stem-cell-enriched side population (SP) cells from human DPCs and periodontal ligament cells (PDLCs), and performed a locked nucleic acid (LNA)-based miRNA array. more...

Organism:

Homo sapiens; Murid gammaherpesvirus 4; Betapolyomavirus hominis; human gammaherpesvirus 4; Human gammaherpesvirus 8; Mus musculus cytomegalovirus 2; Betapolyomavirus macacae; Mus musculus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Human immunodeficiency virus 1; Rattus norvegicus; Human alphaherpesvirus 2; Murid betaherpesvirus 1; JC polyomavirus; Merkel cell polyomavirus

Type:

Non-coding RNA profiling by array

Platform:

GPL11434

4 Samples

Download data: TXT

(Submitter supplied) An 11-point time course study on differentiating embryoid bodies from a murine J1 embryonic stem cell line. The time course includes 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 4 days, 7 days, 9 days and 14 days. Keywords: Time course

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2668 GDS2669

Platforms:

GPL339 GPL340

66 Samples

Download data: CEL, EXP

(Submitter supplied) An 11-point time course study comparing V6.5 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2671 GDS2672

Platforms:

GPL339 GPL340

66 Samples

Download data: CEL, EXP

(Submitter supplied) An 11-point time course study comparing R1 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2666 GDS2667

Platforms:

GPL340 GPL339

66 Samples

Download data: CEL, EXP

Analysis of V6.5 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE3231

33 Samples

Download data: CEL, EXP

Analysis of V6.5 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE3231

33 Samples

Download data: CEL, EXP

Analysis of J1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE3749

33 Samples

Download data: CEL, EXP

Analysis of J1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE3749

33 Samples

Download data: CEL, EXP

Analysis of R1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE2972

33 Samples

Download data: CEL, EXP

Analysis of R1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE2972

33 Samples

Download data: CEL, EXP

(Submitter supplied) Human embryonic stem (hES) cells have the capacities to propagate for extended periods and to differentiate into cell types from all three germ layers both in vitro and in vivo. These characteristics of self-renewal and pluripotency enable hES cells having the potential to provide an unlimited supply of different cell types for tissue replacement, drug screening, and functional genomics studies. The hES-T3 cells with normal female karyotype cultured on either mouse embryonic fibroblasts (MEF) in hES medium (containing 4 ng/ml bFGF) (T3MF) or feeder-free Matrigel in MEF-conditioned medium (supplemented with additional 4 ng/ml bFGF) (T3CM) were found to express very similar profiles of mRNAs and microRNAs, indicating that the unlimited self-renewal and pluripotency of hES cells can be maintained by continuing culture on these two conditions. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL