GEO DataSets

(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray

Organism:

Schizosaccharomyces pombe

1 Series

2 Samples

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(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray Reference: Xue Y, Haas SA, Brino L, Gusnanto A et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 2004 Jan 15;21(1):25-39. PMID: 14745780 Protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). more...

Organism:

Schizosaccharomyces pombe

3 Series

3 Samples

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(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray Protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. more...

Organism:

Schizosaccharomyces pombe

4 Series

14 Samples

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(Submitter supplied) Fission yeast cells undergo sexual differentiation in response to nitrogen starvation. In this process haploid M and P cells first mate to form diploid zygotes, which subsequently enter meiosis and sporulate. Prior to mating, M cells secrete M-factor and P-cells secrete P-factor, and these diffusible mating pheromone can activate a signal transduction pathway in the opposite cell type. The pheromone response orchestrates mating and is also required for entry into meiosis. more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL3424

8 Samples

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(Submitter supplied) DNA microarray technology allows the analysis of genome structure and dynamics at genome-wide scale. Expression microarrays (EMA) contain probes for annotated open reading frames (ORF) and are widely used for the analysis of differential gene expression. By contrast, tiling microarrays (TMA) have a much higher probe density and provide unbiased genome-wide coverage. The purpose of this study was to develop a protocol that would take advantage of the high resolution of TMAs for quantitative measurement of DNA strand-specific differential expression of annotated and non-annotated transcripts. more...

Organism:

Schizosaccharomyces pombe; Schizosaccharomyces pombe 972h-

Type:

Expression profiling by array; Expression profiling by genome tiling array

Platforms:

GPL9672 GPL9671

8 Samples

Download data: CEL

(Submitter supplied) We have found Gcn5 is specifically requried for KCl and CaCl2 mediated stress in Schizosaccharomyces Pombe. We have charaterised the genome wide gene expression responses to KCl stress and show the Gcn5 in involved in the regulation of sebset of stress response genes. Keywords: stress response

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platforms:

GPL3424 GPL3425 GPL3426

12 Samples

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(Submitter supplied) The in-house microarray consists of 167 RT-PCR derived DNA fragments representing 167 ORFs from bovine. The DNA fragments are printed 4 times on the surface of the microarray (four-replicate blocks). The 167 immune and endocrine genes grouped into the following categories: cytokine genes, chemoattractants or chemokines and their respective receptor genes, immune regulatory genes, T-cell associated genes, pattern recognition or innate host defense genes, immunglobin (Ig) or Ig-related genes, complement genes, antigen processing or presenting genes, and genes encoding endocrine hormones or their receptors. more...

Organism:

Arabidopsis thaliana; Bos taurus

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(Submitter supplied) The euryhaline marine yeast Debaromyces hansenii is a model system for the study of genes related to osmotic stress. To study the transcriptional response of this organism to osmotic stress we have used the two color microarray based gene expression analysis of 6211 genes which constitutes the whole genome. Analysis was done at three time points after induction with salt (0.5h, 3h and 6h.) The mRNA level of 64 genes significantly increased at least 3-fold after induction with 2M NaCl whereas that of 45 genes was 3-fold diminished. more...

Organism:

Debaryomyces hansenii

Type:

Expression profiling by array

Platform:

GPL6287

6 Samples

Download data: TXT

(Submitter supplied) In this study, using DNA microarray analysis, we compared the comprehensive expression profiles of two yeast trains, i.e, the previously obtained ethanol-adapted yeast strain and the parental strain as control (FY834), under the ethanol stress condition (YPD medium contained 10% ethanol). As a result, we identified certain genes and functional categories of the genes that are possible involved in growth under the ethanol stress condition.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL7698

2 Samples

Download data: GPR

(Submitter supplied) Genome wide interaction of mediator Keywords: Genome wide interaction of mediator

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array; Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL2789 GPL2795 GPL3592

36 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL1945 GPL4399

15 Samples

Download data: GPR, TIFF, TXT

(Submitter supplied) The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1945

6 Samples

Download data: GPR, TIFF

(Submitter supplied) The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL4399

9 Samples

Download data: TIFF, TXT

(Submitter supplied) S. cerevisiae Y440 Mat a leu2 was grown in YEPD at 28 degrees C with aeration to exponential growth phase and was subjected to a hydrostatic pressure of 50 and 200 MPa for 30 minutes at room temperature. Total RNA was extracted using phenol/chloroform and further precipitated with 3 M sodium acetate / absolute ethanol. Extracted RNA samples were treated for 10 min with 0.5 U of RNAse-free DNAse I / ]g RNA at 37 oC to remove any residual genomic DNA. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2658

2 Samples

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(Submitter supplied) Wild-type cells were cultured at 30 deg and cells were harvested. Total RNAs were purified from 3 populations.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

3 Samples

Download data: CEL

(Submitter supplied) Nylon macroarray (7 x 11 cm) with 6019 single spotted yeast ORF probes. Probes are PCR fragments comprising the whole ORF (5568 cases) or a 1000 bp-fragment from the ORF 3' end (451 cases). Protocol: See Alberola et al. (2004), International Microbiology 7:199-206

Organism:

Saccharomyces cerevisiae

6 Series

77 Samples

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(Submitter supplied) Nylon macroarray (7 x 11 cm) with 6019 single spotted yeast ORF probes. Probes are PCR fragments comprising the whole ORF (5568 cases) or a 1000 bp-fragment from the ORF 3' end (451 cases). Protocol: See Alberola et al. (2004), International Microbiology 7:199-206

Organism:

Saccharomyces cerevisiae

3 Series

42 Samples

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(Submitter supplied) Nylon macroarray (7 x 11 cm) with 6019 single spotted yeast ORF probes. Probes are PCR fragments comprising the whole ORF (5568 cases) or a 1000 bp-fragment from the ORF 3' end (451 cases). Protocol: See Alberola et al. (2004), International Microbiology 7:199-206

Organism:

Saccharomyces cerevisiae

11 Series

166 Samples

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(Submitter supplied) Nylon macroarray (7 x 11 cm) with 6049 single spotted yeast ORF probes. Probes are PCR fragments comprising the whole ORF (5598 cases) or a 1000 bp-fragment from the ORF 3' end (451 cases). Protocol: See Alberola et al. (2004), International Microbiology 7:199-206

Organism:

Saccharomyces cerevisiae

9 Series

83 Samples

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(Submitter supplied) Yeast knockout collection TAG microarrays are an emergent platform for rapid, genome-wide functional characterization of yeast genes. We describe a method for analyzing two-color array data to efficiently represent differential knockout strain representation across two experimental conditions. Using a fully defined spike-in pool, we show that the sensitivity and specificity of this method exceed typical current approaches. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by array

Platform:

GPL1444

5 Samples

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