GEO DataSets

(Submitter supplied) Stenotrophomonas maltophilia is an emerging opportunistic multidrug-resistant pathogen frequently co-isolated with other relevant nosocomial pathogens in respiratory tract infections. S. maltophilia uses the endogenous DSF quorum sensing (QS) system to regulate virulence processes but can also respond to exogenous AHL signals produced by neighboring bacteria. A whole-transcriptome sequencing analysis was performed for S. more...

Organism:

Stenotrophomonas maltophilia K279a

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32376

6 Samples

Download data: CSV, TXT, XLSX

(Submitter supplied) Stenotrophomonas maltophilia is an emerging opportunistic multidrug-resistant pathogen frequently co-isolated with other relevant nosocomial pathogens in respiratory tract infections. S. maltophilia uses the endogenous DSF quorum sensing (QS) system to regulate virulence processes but can also respond to exogenous AHL signals produced by neighboring bacteria. A whole-transcriptome sequencing analysis was performed for S. more...

Organism:

Stenotrophomonas maltophilia K279a

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32370

14 Samples

Download data: CSV, TXT, XLSX

(Submitter supplied) In this study, we used host and bacterial RNA sequencing to evaluate the impact of dual species infections with S. maltophilia and P. aeruginosa on CF disease progression. To evaluate S. maltophilia transcript profiles we used a recently described method for selective capture of bacterial mRNA transcripts with strain specific RNA probes.

Organism:

Mus musculus; Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32792 GPL32791 GPL13112

26 Samples

Download data: TXT, XLSX

(Submitter supplied) Transcriptomic analysis of S. maltophilia D457 after one-hour induction with the antibiotic fosfomycin, the intermediate metabolites phosphoenolpyruvate or glyceraldehyde-3-phosphate

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30504

13 Samples

Download data: TXT

(Submitter supplied) Stenotrophomonas maltophilia is an emerging multidrug resistance opportunistic pathogen affecting immunocompromised and hospitalized patients. S. maltophilia is an environmental bacterium which adapts to human body and causing infection. S. rhizophilia, a non-pathogenic and phylogenetic neighbour of S. maltophilia is unable to grow at human body temperature. Thus, to understand molecular mechanism underlying the adaptation of S. more...

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23835

6 Samples

Download data: TXT

(Submitter supplied) Fosfomycin is a bactericidal antibiotic, analogous to phosphoenolpyruvate (PEP) that exerts its activity by inhibiting the activity of MurA. This enzyme catalyzes the first step of peptidoglycan biosynthesis, the transfer of enolpyruvate from PEP to uridine- diphosphate-N-acetylglucosamine. Fosfomycin is increasingly used in the last years, mainly for treating infections caused by Gram-negative multidrug resistant bacteria as Stenotrophomonas maltophilia, an opportunistic pathogen characterized by its low susceptibility to antibiotics of common use. more...

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25680

5 Samples

Download data: XLS

(Submitter supplied) We characterized the transcriptional responses of three S. maltophilia strains during exposure to synthetic CF sputum media (SCFM2) to gain insight into how this organism interacts with the host in the CF lung. These efforts led to the identification of 881 transcripts differentially expressed by all three strains, many of which reflect different metabolic pathways used by S. maltophilia in sputum, and altered stress responses. more...

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23835

12 Samples

Download data: CSV

(Submitter supplied) We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes.

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25680

7 Samples

Download data: XLSX

(Submitter supplied) The interactions between Gram-negative respiratory pathogens and the host environment at the site of infection largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into this ecological transition, we characterized the transcriptional responses of Pseudomonas aeruginosa PA14, Burkholderia thailandensis E264, Klebsiella pneumoniae MGH 78578, and Stenotrophomonas maltophilia K279A exposed to purified pulmonary surfactant (Survanta) through microarrays. more...

Organism:

Burkholderia thailandensis E264; Klebsiella pneumoniae MGH 78578; Pseudomonas aeruginosa PA14; Stenotrophomonas maltophilia K279a

Type:

Expression profiling by array

Platform:

GPL24615

8 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) We calculated half-life values of mRNAs quantified by RNA-Seq by a suitable method of normalization. We determined the half-lives of more than 2200 mRNAs in the Stenotrophomonas maltophilia D457 wild-type strain and in an isogenic RNase G deficient mutant. Median half-lives were 2,74 and 3 min in the wild-type and the rng-deficient mutant respectively. We found an overall enhancement of half-life times of mRNAs when the gene encoding RNase G is lacking, showing that many RNAs are targets of RNase G in S. more...

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23835

8 Samples

Download data: TXT

(Submitter supplied) Stenotrophomonas maltophilia K279a diverges into subpopulations with distinct but reversible phenotypes of small and big colonies when challenged with ampicillin. This observation is consistent with the formation of long cell chains during exponential growth phase and the occurrence of mainly coccoid– or rod-shaped cells in liquid media. Further, scanning electron micrographs of SMK279a revealed that cells formed gigantic outer membrane vesicles in response to β-lactam treatment. more...

Organism:

Stenotrophomonas maltophilia K279a

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20774

10 Samples

Download data: WIG

(Submitter supplied) Stenotrophomonas maltophilia is an important opportunistic pathogen affecting primarily hospitalized and immuno-compromised hosts. We constructed an hfq deletion mutant (Delta-hfq) of S. maltophilia, and compared the behaviour of wild-type and Delta-hfq S. maltophilia cells in a variety of assays. Differential RNA sequencing analysis (dRNA-seq) of RNA isolated from S. maltophilia wild-type and Delta-hfq strains showed that Hfq regulates expression of genes encoding flagellar and fimbrial components, transmembrane proteins, as well as enzymes involved in different metabolic pathways. more...

Organism:

Stenotrophomonas maltophilia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15858

4 Samples

Download data: WIG

(Submitter supplied) see manufacturer's web site

Organism:

Burkholderia thailandensis E264; Stenotrophomonas maltophilia K279a; Pseudomonas aeruginosa PA14; Klebsiella pneumoniae MGH 78578

1 Series

8 Samples

Download data: CDF, CSV, GIN, TXT