GEO DataSets

(Submitter supplied) Sickle cell disease is the most common genetic disorder in African-Americans. The opioid analgesic, morphine, has long been the treatment for the severe pain associated with this disease. Here we reveal that the opioid antagonist, naloxone, possesses potent analgesic activity in two strains of sickle cell mice (NY1DD and hBERK1) and not in their respective controls (ICR-CD1 and C57BL/6J) when administered by three parenteral routes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4464

12 Samples

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(Submitter supplied) Sickle cell disease is the most common genetic disorder in African-Americans. The opioid analgesic, morphine, has long been the treatment for the severe pain associated with this disease. Here we reveal that the opioid antagonist, naloxone, possesses potent analgesic activity in two strains of sickle cell mice (NY1DD and hBERK1) and not in their respective controls (ICR-CD1 and C57BL/6J) when administered by three parenteral routes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2826

Platform:

GPL4464

6 Samples

Download data: GPS

Analysis of brains of NY1DD transgenic animals treated with naloxone. The NY1DD strain is a model for sickle cell anemia. Naloxone is an opioid antagonist that possesses potent analgesic activity in NY1DD animals. Results provide insight into the mechanism of action of naloxone in this model.

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 2 strain sets

Platform:

GPL4464

Series:

GSE6578

6 Samples

Download data: GPS

(Submitter supplied) Basic protocol for generation of mouse microarrays i) Obtain cDNA library (1) thousands of bacterial stocks in 96-well plates (2) each bacterial stock contains a standard eukaryotic expression plasmid that contains a unique stretch of mouse cDNA inserted in the multiple cloning site ii) Replicate the library iii) PCR amplify each cDNA clone in 96 well reactions iv) Check PCR reactions on an agarose gel v) Purify PCR reactions with a Millipore filter vi) Elute purified DNA with 3XSSC (spotting buffer) vii) Spot DNA onto glass slides using a DNA spotting robot

Organism:

Mus musculus

1 DataSet

2 Series

12 Samples

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