GSM109319[Accession] - GEO DataSets

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Items: 3

Select item 2000048721.

Response of Lactococcus lactis to isoleucine starvation

(Submitter supplied) For the first time in Lactococcus lactis, amino acid starvation response was characterized. The natural imposition of isoleucine starvation, by its consumption during growth, associated to transcript profiling, allowed defining exhaustively this stress stimulon. It consisted of a general induction of nitrogen metabolism (amino acid biosynthesis and transport, proteolytic system and proteases), a strong repression of genes encoding major physiological activities (translation, transcription, carbon metabolism, purine and pyrimidine biosynthesis and fatty acid metabolism) and the induction of unexpected cross responses to acid, osmotic and oxidative stresses. more...

Organism:: Lactococcus lactis

Type:: Expression profiling by array

Platform:: GPL3782
12 Samples

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Accession:: GSE4872

ID:: 200004872

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000037822.

INSA Lactococcus lactis IL1403 (lot de PCR n°1)

(Submitter supplied) nylon membranes positively charged 8*12 cm Protocol: Amplicons (PCR) were designed and amplified by Eurogentec. A mean length of 535 bp and final concentrations between 40 and 380 µg/l were obtained. PCR were diluted in DMSO 50 % in 384 wells spot plates and spotted (4 deposits/ spot) in duplicate on positively charged nylon membranes (Roche) with a Biogrid spotter (Biorobotics) with a 4*4 pattern.