GEO DataSets

(Submitter supplied) Protein synthesis is a major energy-consuming process of the cell, which requires controlled production and turnover of ribosomes. While the last years have seen major advances in our understanding of ribosome biogenesis, structural insight into the degradation of ribosomes has been lacking. Here we present native structures of two distinct small ribosomal 30S subunit degradation intermediates associated with the 3’ to 5’ exonuclease, RNase R. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21373

9 Samples

Download data: BEDGRAPH

(Submitter supplied) We obtained whole genome sequencing data as a measure of chromosome replication to see if deletion or overexpression of ccrZ altered replication initiation

Organism:

Bacillus subtilis

Type:

Other

Platform:

GPL21373

9 Samples

Download data: TXT

(Submitter supplied) We obtained whole genome sequencing data as a measure of chromosome replication to see if HU inhibits replication compared to vehicle control.

Organism:

Bacillus subtilis

Type:

Other

Platform:

GPL21373

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus subtilis

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL21373 GPL19910 GPL29515

42 Samples

Download data

(Submitter supplied) During steady-state cell growth, individual enzymatic fluxes can be directly inferred from growth rate by mass conservation, but the inverse problem remains unsolved. Perturbing the flux and expression of a single enzyme could have pleiotropic effects that may or may not dominate the impact on cell fitness. Here, we quantitatively dissect the molecular and global responses to varied expression of translation termination factors (peptide release factors, RFs) in the bacterium Bacillus subtilis. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL21373 GPL24109 GPL18561

107 Samples

Download data: WIG

(Submitter supplied) These experiments were designed to quantify depletion of rRNA sequencing reads from bacterial RNA-seq libraries and verify that mRNA sequencing reads were not altered. Specifically, we tested an rRNA depletion method using custom-designed biotinylated oligonucleotides and compared these results to undepleted (total RNA) libraries and libraries made with the previously-available Ribo-Zero kit (Illumina).

Organism:

Escherichia coli; Bacillus subtilis; Caulobacter vibrioides

Type:

Expression profiling by high throughput sequencing

4 related Platforms

12 Samples

Download data: CSV

(Submitter supplied) Using RNA-seq, we cataloged messenger RNAs in highly purified dormant Bacillus subtilis spores prepared either on plates or in liquid. Almost all of the most abundant spore mRNAs are encoded by genes expressed only in the developing spore late in sporulation under control of the forespore-specific RNA polymerase sigma factor, sG. Given the levels of the ~40 most abundant mRNAs in dormant spores, we calculated the great majority of the low abundance mRNAs can be present in only a small fraction of the spore population.

Organism:

Bacillus subtilis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21373

9 Samples

Download data: TXT

(Submitter supplied) The narrow-specificity endoribonuclease RNase III and the 5’ exonuclease RNase J1 have been recently found to be not essential in the Gram-positive model organism, Bacillus subtilis. In this study, we performed a global analysis of internal 5’ ends that are generated or acted upon by these enzymes. An RNA-Seq protocol known as PARE (Parallel Analysis of RNA Ends) was used to capture 5’ monophosphorylated RNA ends in ribonuclease wild-type and mutant strains. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18561 GPL21373

7 Samples

Download data: TXT