8D2K: Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 2)

PDB ID: 8D2KDownload
MMDB ID: 240121
PDB Deposition Date: 2022/5/30
Updated in MMDB: 2023/12
Experimental Method:
electron microscopy
Resolution: 2.43  Å
Source Organism:
Acidothermus cellulolyticus 11B▼
Similar Structures:
Biological Unit for 8D2K: hexameric; determined by author
Molecular Components in 8D2K
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease, Csn1 Family
Molecule annotation
Nucleotides(5 molecules)
1
Single Guide RNA (102-mer)
Molecule annotation
1
DNA Target Strand (5'-d(*ap*gp*cp*tp*tp*gp*gp*tp*gp*tp*ap*tp*a)-3')
Molecule annotation
1
DNA Target Strand (5'-d(p*cp*cp*ap*gp*gp*ap*tp*cp*tp*tp*gp*cp*cp*ap*tp*cp*cp*tp*ap*cp*cp*tp*cp*t)-3')
Molecule annotation
1
DNA Non-target Strand (5'-d(p*tp*ap*tp*ap*cp*ap*cp*cp*ap*ap*gp*cp*t)-3')
Molecule annotation
1
DNA Non-target Strand (5'-d(p*ap*gp*a)-3')
Molecule annotation
Chemicals and Non-standard biopolymers (10 molecules)
1
10
MAGNESIUM IONMAGNESIUM ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303