mRNA-Seq of untreated bacteria - SRA

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SRX12891316: mRNA-Seq of untreated bacteria
1 ILLUMINA (Illumina NovaSeq 6000) run: 4.2M spots, 1.3G bases, 381Mb downloads

Design: RNA integrity was checked by 2% gel electrophoresis, run at 100V for 1h, and checked by Agilent 5400 Fragment Analyzer System. RNA concentration was measured by NanoDrop, spectrophotometrically. RNA libraries were prepared using Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina, San Diego, CA, USA) kit. Sequencing was done by Illumina NovaSeq 6000 platform as paired-end (PE) 2x150bp layout. 4-5M reads per sample, about 1.5Gbp data were produced.

Submitted by: Bilecik Seyh Edebali University

Study: Escherichia coli Transcriptome: Untreated; 1.0%, v/v DMSO treated; 2.5%, v/v DMSO treated

PRJNA777047 • SRP372891 • All experiments • All runs

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The effect of low-dose dimethyl sulfoxide (DMSO) concentrations (%1.0 and %2.5, v/v), which do not affect the viability, on gene bacterial expression was investigated using RNA-seq in comparison with the untreated samples.

Sample: Untreated

SAMN22830864 • SRS10833847 • All experiments • All runs

Organism: Escherichia coli ATCC 8739

Library:

Name: 1

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 4.2M spots, 1.3G bases, 381Mb

Run	# of Spots	# of Bases	Size	Published
SRR16690734	4,170,056	1.3G	381Mb	2022-07-29

ID:: 17565887

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