DNA-Seq of S. saprophyticus: milker's hand - SRA

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SRX6867969: DNA-Seq of S. saprophyticus: milker's hand
1 PACBIO_SMRT (PacBio RS II) run: 92,703 spots, 923.7M bases, 605.3Mb downloads

Design: The construction of SMRTbell libraries suitable for generating high accuracy long reads on the Sequel Systems requires 15 g of high molecular weight genomic DNA (gDNA). The DNA is sheared to a mode of 15 kb using Diagenodes Megaruptor. SMRTbell libraries are prepared from sheared gDNA using PacBios SMRTbell Template Prep Kit 1.0. SMRTbell libraries are then size-fractionated using Sage Sciences SageELF. The fractions suitable for sequencing have insert sizesfrom 10 kb to 15 kb.

Submitted by: Sao Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal

Study: Staphylococcus saprophyticus strain:1A Genome sequencing

PRJNA482667 • SRP222367 • All experiments • All runs

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Elucidating the virulence and antimicrobial resistance mechanisms of Staphylococcus spp. associated with bubaline mastitis, through "single-molecule, real-time sequencing" and comparative genomics

Sample:

SAMN09710868 • SRS5405402 • All experiments • All runs

Organism: Staphylococcus saprophyticus

Library:

Name: SS1A

Instrument: PacBio RS II

Strategy: WGS

Source: GENOMIC

Selection: RT-PCR

Layout: SINGLE

Runs: 1 run, 92,703 spots, 923.7M bases, 605.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR10140042	92,703	923.7M	605.3Mb	2019-09-19

ID:: 9038705

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