Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA isolated by mechanical shaking (5 min) with sterile glass beads using the RNeasy Qiagen kit (Qiagen) according to the manufacturer’s protocol. Residual DNA was depleted from RNA samples using the RNAqueous-4PCR kit according to the manufacturer’s instructions and ribosomal RNAs depleted of using Illumina RiboZero rRNA removal kit (Illumina, San Diego, CA). Samples were prepared with TrueSeq Stranded mRNA HT library preparation kit (Illumina, San Diego, CA) and sequenced using Illumina HiSeq 2000 sequencing machine with approximately 10-15 million reads per sample.