show Abstracthide AbstractFecal samples from 18 poultry farms (twelve layers and six broilers) in Faisalabad, Pakistan were analyzed using 16S rRNA gene sequencing of the V3-V4 region to evaluate bacterial composition. Fecal samples were collected by sterile forceps from 18 different commercial farms: broilers (n = 6) and layer (n = 12) across the district of Faisalabad, Pakistan. Three fecal samples per farm were collected. DNA was extracted with the QIAamp Fast DNA Stool Mini Kit.