show Abstracthide AbstractThis study aims to investigate the microbial diversity and function of the gut microbiome in village chickens from different regions in South Africa. By utilizing advanced sequencing technologies like 16S rRNA gene sequencing and shotgun metagenomics, researchers aim to characterize the bacterial communities present in the chicken gut, identifying potential pathogens and beneficial microbes. Understanding the gut microbiome is crucial as it plays a significant role in animal health, productivity, and resilience to diseases. This research can provide valuable insights into the factors influencing the gut microbial composition of village chickens, which can inform strategies for improving their health and well-being. Additionally, the study can shed light on potential zoonotic risks associated with the microbial communities present in these animals.