RAD-seq of Pacific Oyster: Molluscan Broodstock Program, Oregon State... - SRA

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SRX25641102: RAD-seq of Pacific Oyster: Molluscan Broodstock Program, Oregon State University
1 ION_TORRENT (Ion Torrent Proton) run: 1.9M spots, 154.2M bases, 106.5Mb downloads

Design: DNA was extracted from adductor muscle sections of Crassostrea gigas, purified and quantified by fluorometry, then normalized to 10 ng/ul and used as an input to double-digest restriction site associated (RAD)-sequencing library preparation. Genomic DNA was digested using NsiI and MspI cutting enzymes, and size selected. Samples were individually barcoded and multiplexed at 32 individuals per chip. Multiplexed libraries were sequenced on an Ion Torrent Proton. Fastq data were trimmed for adapters and low quality sequences, then demultiplexed and truncated to a consistent size (80 bp), then uploaded to NCBI.

Submitted by: Vancouver Island University

Study: Selection of Pacific oysters using an OsHV-1 resistance marker has no influence on susceptibility to Vibrio aestuarianus

PRJNA1145972 • SRP525048 • All experiments • All runs

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Reduced representation sequencing (double-digest restriction site-associated DNA sequencing) dataset to support a genome-wide association study of survival to a Vibrio aestuarianus infection in a selectively-bred population of Pacific oyster for viral resistance.

Sample: 188

SAMN43080898 • SRS22285365 • All experiments • All runs

Organism: Magallana gigas

Library:

Name: F115-Dead_188

Instrument: Ion Torrent Proton

Strategy: RAD-Seq

Source: GENOMIC

Selection: Reduced Representation

Layout: SINGLE

Runs: 1 run, 1.9M spots, 154.2M bases, 106.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR30174771	1,927,329	154.2M	106.5Mb	2024-11-21

ID:: 34655679

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