show Abstracthide AbstractWe collected data from 13 patients with tumors in the biliary tract cancers invading the bile duct and subsequently underwent surgical resection: 7 cholangiocarcinomas, 1 gallbladder carcinoma, 3 ampullary carcinomas, 1 pancreatic cancer, and 1 intraductal papillary mucinous carcinoma. Whole-exome sequencing-based extracted DNA from resected samples revealed pathogenic alterations of TP53, ABCA8, BRCA2, FGFR2, APC, SMAD4 and NRAS.