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SRX23775965: GBS of T. plicata adults and juveniles
1 ILLUMINA (Illumina NovaSeq 6000) run: 2.6M spots, 773M bases, 248.4Mb downloads

Design: The library was constructed with PstI/MspI enzyme combination (NEB, Ipswich, MA, USA) and the Illumina TruSeq HT Library Preparation Kit (Illumina, Inc. San Diego, CA, USA) (Figure 2), retaining on the fragments above 300bp from a fragment selection step. Sequencing was performed on an Illumina NovaSeq 6000 PE150 platform (Illumina Inc. San Diego, CA, USA), and 150 bp paired-end raw files were obtained.
Submitted by: University of Oxford
Study: Genetic Diversity in Thuja plicata planted stands
show Abstracthide Abstract
This work is based on the hypothesis that low standing genetic diversity may be present in T. plicata in woodlands being converted to a Continuous Cover Forestry (CCF) regime, together with human selective harvesting selection and logging of individual trees may result in naturally-regenerated juveniles with an even narrower genetic base or a low potential to adapt to changing conditions. In this project we looked at planted T. plicata trees from several woodlands and conducted an SNP discovery step by using GBS and subsequently selected a subset of informative SNP markers for larger scale genotyping. The overarching goal of this study is to assess the differences in genetic diversity between planted trees and their offspring considering four different T. plicata plantations in the UK.
Sample:
SAMN40149471 • SRS20600903 • All experiments • All runs
Organism: Thuja plicata
Library:
Name: S4PR35_GBS_psti_mspi_ILLUMINA_PAIREND
Instrument: Illumina NovaSeq 6000
Strategy: GBS
Source: GENOMIC
Selection: Restriction Digest
Layout: PAIRED
Runs: 1 run, 2.6M spots, 773M bases, 248.4Mb
Run# of Spots# of BasesSizePublished
SRR281450692,620,308773M248.4Mb2024-04-30

ID:
32069197

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