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SRX22478524: GSM7889093: cra2, root, satiety, rep2; Medicago truncatula; miRNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 10.1M spots, 217.8M bases, 98.7Mb downloads

External Id: GSM7889093_r1
Submitted by: IPS2, CNRS
Study: miRNAs regulated systemically by nitrogen availability in shoots and roots of Medicago truncatula
show Abstracthide Abstract
Using a dedicated split-root approach, we identified miRNAs regulated systemically by nitrogen availability in both shoots and roots of the Medicago truncatula model legume, depending on the CRA2 pathway, highlighting the phosphate-related miR399. Overall design: small RNA-seq of distant split-root or shoots of medicago truncatulla control (A17) or cra2 mutant where local split-roots were subjected to nitrogen satiety or deficitconducted in triplicate.
Sample: cra2, root, satiety, rep2
SAMN38194838 • SRS19492163 • All experiments • All runs
Library:
Name: GSM7889093
Instrument: Illumina NovaSeq 6000
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNAs were extracted using the miRNeasy mini kit (Qiagen). Libraries were prepared using the NEXTFLEX Small RNA-seq kit v3 (PerkinElmer) following the manufacturer's instructions. The resulting barcoded library was size-selected (137-187 bp) using a Pippin HT (SAGE Science) with a 3 % agarose cassette (#HTG3004).
Runs: 2 runs, 10.1M spots, 217.8M bases, 98.7Mb
Run# of Spots# of BasesSizePublished
SRR267799162,506,50754.1M25Mb2024-07-17
SRR267799177,569,047163.7M73.7Mb2024-07-17

ID:
30464313

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