RNA-seq of Nostoc punctiforme: week 2 of growth without Cl - SRA

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SRX17027291: RNA-seq of Nostoc punctiforme: week 2 of growth without Cl
1 ILLUMINA (Illumina HiSeq 4000) run: 80.6M spots, 24.2G bases, 6.9Gb downloads

Design: Nostoc punctiforme ATCC 29133 was cultured in BG-11 medium with or without chloride. After 2 weeks (active growth) or 4 weeks (nearing stationary phase), the cultures were quickly homogenized by pipetting, and 1 mL was transferred to a 1.7-mL microcentrifuge tube. The tube was centrifuged for 30 s at 16200 g. The supernatant was carefully aspirated, and the pellet was immediately frozen in liquid nitrogen. Culture harvesting was staggered so that the total time between homogenization and snap freezing was less than 2 min. Samples were stored at -70 °C until submission to the standard RNA-seq service provided by Genewiz (Azenta Life Sciences). RNA isolation was performed using an RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was depleted using a QIAseq FastSelect kit (Qiagen) and libraries were prepared using a NEBNext Ultra II RNA Library Prep Kit (New England Biolabs).

Submitted by: Harvard University

Study: Response of Nostoc punctiforme ATCC 29133 to halide depletion

PRJNA868493 • SRP390980 • All experiments • All runs

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The goal of this study is to characterize the response of the cyanobacterium Nostoc punctiforme ATCC 29133 to halide depletion.

Sample:

SAMN30247640 • SRS14612156 • All experiments • All runs

Organism: Nostoc punctiforme PCC 73102

Library:

Name: noClweek2B

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 80.6M spots, 24.2G bases, 6.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR21010784	80,643,167	24.2G	6.9Gb	2023-09-15

ID:: 23814517

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