show Abstracthide AbstractSummary: We generated RNA-sequencing data from lymphoblastoid cell lines derived from 731 unrelated individuals from the 1000 Genomes Project. Samples are roughly evenly distributed among 26 globally diverse subpopulations. This dataset provides a broad view of human gene expression diversity, while serving as a resource for discovery of genetic variation that influences gene expression and splicing in this globally diverse cohort. Design: All cell lines were obtained from the Coriell Institute for Medical Research with consent to deposit sequencing reads and sample metadata. RNA extraction, RNA-Seq PolyA selection library preparation, and sequencing was performed by GENEWIZ (Azenta Life Sciences). 24 samples were sequenced in triplicate.