RNA-Seq of Gluconacetobacter diazotrophicus (GD-F) inoculated Arabido... - SRA

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SRX12495567: RNA-Seq of Gluconacetobacter diazotrophicus (GD-F) inoculated Arabidopsis thaliana: leaf
1 ILLUMINA (Illumina HiSeq 2000) run: 22.2M spots, 2.2G bases, 876.2Mb downloads

Design: Total RNA was isolated sample tissues using the RNeasy Plant Mini Kit (Qiagen, Germany). The quantity, quality, and integrity of the total RNA were examined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Magnetic beads with Oligo (dT) were used to isolate mRNA from the total RNA. The isolated mRNA was cut into short fragments under high temperatures. After that, double-stranded cDNA was synthesized using the SuperScript Synthesis Kit (Invitrogen, USA) with random hexamer primers (Illumina). The cDNA fragments were purified and washed for terminal reparation and poly(A) addition. The short fragments were connected with adapters. The fragments (200 pb) were PCR-amplified for cDNA library construction

Submitted by: Embrapa Informatica Agropecuaria

Study: Arabidopsis thaliana response to endophytic colonization by Gluconacetobacter diazotrophicus

PRJNA768887 • SRP340155 • All experiments • All runs

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Gluconacetobacter diazotrophicus is a plant growth-promoting bacterium that colonizes several crop species, but the molecular mechanisms activated during such interaction remain poorly understood. This work uses comparative transcriptomics to characterize the molecular pathways activated during the association of G. diazotrophicus with Arabidopsis thaliana.

Sample: Inoculated Leaf 3

SAMN22069556 • SRS10458508 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: FolhaGDA3

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 22.2M spots, 2.2G bases, 876.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR16213512	22,157,011	2.2G	876.2Mb	2024-11-01

ID:: 16938266

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