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SRX8892954: Target enrichment of stripe locus in East African cichlids
1 ILLUMINA (Illumina HiSeq 2500) run: 1.4M spots, 412.6M bases, 217.8Mb downloads

Design: Target capture data was produced using customized 120 nt baits with ~3x flexible tiling density by MYBaits for the 270 kb interval around agrp2/asip2b. Baits were designed from the Pundamilia nyererei genome which was curated by filling gaps of the genome assembly using Sanger sequencing reads so that the modified genome now contains the stripe interval (https://datadryad.org/stash/dataset/doi:10.5061/dryad.bnzs7h467). For library preparation, we used the Illumina TruSeq Nano HT Library preparation kit (Illumina Inc.) following the manufacturers guidelines. For the baits, we followed the MYBaits manual v3 (http://www.mycroarray.com/pdf/MYbaits-manual-v3.pdf) and hybridized the probes at 65C for 22 hours.
Submitted by: University of Konstanz
Study: Stripe pattern divergence in cichlid fish adaptive radiations
show Abstracthide Abstract
Convergent evolution of melanic horizontal stripes has been linked to a single locus harboring the gene agouti-related peptide 2 (agrp2). However, where and when the causal variants underlying this trait evolved and how they drove phenotypic divergence remained unknown. To test the alternative hypotheses of standing genetic variation versus de novo mutations (independently originating in each radiation), we searched for shared signals of genomic divergence at the agrp2 locus.
Sample: SU0517B_83
SAMN15685487 • SRS7151975 • All experiments • All runs
Library:
Name: SU0517B_83
Instrument: Illumina HiSeq 2500
Strategy: Targeted-Capture
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 1.4M spots, 412.6M bases, 217.8Mb
Run# of Spots# of BasesSizePublished
SRR123945491,366,068412.6M217.8Mb2020-08-05

ID:
11560926

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