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ERX2789703: Illumina HiSeq 2000 sequencing
1 ILLUMINA (Illumina HiSeq 2000) run: 8.5M spots, 433.3M bases, 247.3Mb downloads

Submitted by: ROTHAMSTED RESEARCH
Study: Analysis of small RNA silencing in Zymoseptoria tritici – wheat interactions
show Abstracthide Abstract
Deep sequencing of small RNA preparations from Zymoseptoria tritici isolate IPO323 cultured in vitro (in Czapek-Dox Broth, CDB) and those from the infected wheat cv. Bobwhite leaf tissues sampled at the four different time points corresponding to the four critical phases of Septoria tritici blotch disease development, namely 4 dpi (asymptomatic phase), 9 dpi (transition to necrotrophic stage), 13 dpi (necrotrophic phase), and 21 dpi (profuse asexual sporulation) was carried out on an Illumina HiSeq 2000 using 50 cycle single end reads. Deep sequencing of small RNA preparations from mock-inoculated wheat cv. Bobwhite leaves sampled at 4 dpi, 9 dpi, 13 dpi, and 21 dpi and from healthy, untreated 17 days old wheat cv. Bobwhite leaves was also carried out using the same sequencing technology.
Sample: small RNA samples
SAMEA4883108 • ERS2702114 • All experiments • All runs
Library:
Name: L002_R1_001
Instrument: Illumina HiSeq 2000
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Runs: 1 run, 8.5M spots, 433.3M bases, 247.3Mb
Run# of Spots# of BasesSizePublished
ERR27776268,495,320433.3M247.3Mb2019-06-06

ID:
8015379

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