WGS of Escherichia coli, strain NM84_B9-29: murine gut isolate - SRA

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SRX5823839: WGS of Escherichia coli, strain NM84_B9-29: murine gut isolate
1 ILLUMINA (Illumina NovaSeq 6000) run: 33.4M spots, 9.3G bases, 2.8Gb downloads

Design: Sequencing performed by Dr. Brian Yu at the Genomics Platform of the Chan Zuckerberg Biohub. Nextera XT kits were used to make the libraries with CZ Biohubs custom designed adapters - 12 bp index sequences on each side of the library and are dual unique so that each sequence only appears once. The libraries were made in 384 well plates in 4 uL of total volume. Genomic DNA was diluted to 0.18 ng/uL and 0.4 uL was added to the library prep reaction using Mosquito HTS from TTP. 0.5 uL of the library was pooled for sequencing. Sequencing was performed on a NovaSeq S4 flow cell with the run configuration 140 (12) (12) 140, so thats 2x140 bps with dual 12 bp index sequences.

Submitted by: University of Toronto

Study: Genome sequencing and assembly - library of cultured bacterial species from laboratory mouse gut microbiota from the University of Toronto

PRJNA474907 • SRP193474 • All experiments • All runs

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Bank of bacterial mono-isolates from laboratory mouse gut microbiota

Sample:

SAMN11366402 • SRS4751923 • All experiments • All runs

Organism: Escherichia coli

Library:

Name: NM84_B9-29

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 33.4M spots, 9.3G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR9047311	33,378,044	9.3G	2.8Gb	2019-05-13

ID:: 7829557

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