Shotgun sequencing of the genome of Eutypa lata (isolate UCREL1) - SRA

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SRX220831: Shotgun sequencing of the genome of Eutypa lata (isolate UCREL1)
1 ILLUMINA (Illumina HiSeq 2000) run: 35.1M spots, 7G bases, 4.5Gb downloads

Design: Genomic DNA was extracted mycelia growing on PDA plate. A single DNA library was prepared from 5 ug of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA). Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent).

Submitted by: UC Davis

Study: Eutypa lata UCREL1 Genome sequencing and assembly

PRJNA187490 • SRP018314 • All experiments • All runs

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Eutypa lata is the causal agent of Eutypa dieback of grapevines (Vitis vinifera). Eutypa symptoms include: 1) a dark wedge-shaped area in cross-sections of the trunk and cordons; 2) external cankers, 2) stunted shoots; and; 4) reduced yields. Plant hosts include lemon, apple, pear, almond, and pistachio.

Sample: Eutypa lata Isolate UCREL1

SAMN01906717 • SRS388387 • All experiments • All runs

Organism: Eutypa lata UCREL1

Library:

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 35.1M spots, 7G bases, 4.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR654028	35,139,568	7G	4.5Gb	2013-04-24

ID:: 308336

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