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ERX005931: Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation
1 ILLUMINA (Illumina Genome Analyzer II) run: 7.6M spots, 1.2G bases, 733.3Mb downloads

Design: Zebrafish gene 3 prime end pull down for genome annotation
Submitted by: The Wellcome Trust Sanger Institute (SC)
Study: Zebrafish gene 3 prime end pull down for genome annotation
Sample: ZF_ovary_sample1
SAMEA708832 • ERS000090 • All experiments • All runs
Organism: Danio rerio
Library:
Name: Zebrafish adult ovary mRNA 3' end
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: 20 ug of total RNA was fragmented using RNA Fragmentation Reagent (Ambion) for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl. RNA was annealed to the oligo stBPM1polyT22 (biotin-GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN) and bound to streptavidin magnetic beads. After washing by pull down on a magnet, the bound RNA was reverse transcribed with SuperScript II (Invitrogen) and a second strand synthesised with DNA polymerase I (Promega) and RNase H (NEB). After further washing, the double strand cDNA was released from the beads with BpmI (NEB). The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.
Experiment attributes:
Experimental Factor: DEVELOPMENTAL_STAGE: adult
Experimental Factor: ORGANISM_PART: ovary
Experimental Factor: SEX: female
Runs: 1 run, 7.6M spots, 1.2G bases, 733.3Mb
Run# of Spots# of BasesSizePublished
ERR0155667,567,0611.2G733.3Mb2010-09-14

ID:
31874

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