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    PCAP1 plasma-membrane associated cation-binding protein 1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 827773, updated on 18-Sep-2024

    Summary

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    Official Symbol
    PCAP1
    Official Full Name
    plasma-membrane associated cation-binding protein 1
    Primary source
    TAIR:AT4G20260
    Locus tag
    AT4G20260
    See related
    Araport:AT4G20260
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ARABIDOPSIS THALIANA PLASMA-MEMBRANE ASSOCIATED CATION-BINDING PROTEIN 1; ATPCAP1; F1C12.180; F1C12_180; MDP25; microtubule-destabilizing protein 25; plasma-membrane associated cation-binding protein 1
    Summary
    Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels.
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    Genomic context

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    See PCAP1 in Genome Data Viewer
    Location:
    chromosome: 4
    Exon count:
    5
    Sequence:
    Chromosome: 4; NC_003075.7 (10940414..10943561)

    Chromosome 4 - NC_003075.7Genomic Context describing neighboring genes Neighboring gene cytochrome P450, family 71, subfamily A, polypeptide 28 Neighboring gene cytochrome P450, family 71, subfamily A, polypeptide 27 Neighboring gene uncharacterized protein Neighboring gene ncRNA Neighboring gene miscRNA Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003075.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein. Vallet A, et al. J Hazard Mater, 2023 Mar 15. PMID 36608581
    2. Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane. Ide Y, et al. J Exp Bot, 2007. PMID 17264065
    3. A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P(3) and PtdIns(3,5)P(2). Nagasaki N, et al. FEBS J, 2008 May. PMID 18397324
    4. A plasma membrane-associated protein of Arabidopsis thaliana AtPCaP1 binds copper ions and changes its higher order structure. Nagasaki-Takeuchi N, et al. J Biochem, 2008 Oct. PMID 18664522
    5. MDP25, a novel calcium regulatory protein, mediates hypocotyl cell elongation by destabilizing cortical microtubules in Arabidopsis. Li J, et al. Plant Cell, 2011 Dec. PMID 22209764, Free PMC Article

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein.
      Title: The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein.
    2. The plasma membrane-associated Ca(2+) -binding protein, PCaP1, is required for oligogalacturonide and flagellin-induced priming and immunity.
      Title: The plasma membrane-associated Ca(2+) -binding protein, PCaP1, is required for oligogalacturonide and flagellin-induced priming and immunity.
    3. contributes to hydrotropism through a MIZ1-independent pathway or as one of the upstream components that transduce water potential signals to MIZ1
      Title: Plasma Membrane-Associated Ca2+-Binding Protein PCaP1 is Involved in Root Hydrotropism of Arabidopsis thaliana.
    4. PCaP1 is involved in the stomatal movement, especially closure process, in leaves and response to excessive copper in root and leaf as a mineral nutrient as a physiological role.
      Title: A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsis thaliana: stable association with plasma membrane and partial involvement in stomata closure.
    5. MICROTUBULE-DESTABILIZING PROTEIN25 (MDP25) has the actin filament-severing activity of an actin binding protein.
      Title: Arabidopsis microtubule-destabilizing protein 25 functions in pollen tube growth by severing actin filaments.
    6. MDP25 destabilizes microtubules and exerts its effect on microtubules as a result of transient elevation of cytosolic calcium levels. [MDP25]
      Title: Characterization of the role of calcium in regulating the microtubule-destabilizing activity of MDP25.
    7. potyviral P3N-PIPO interacts specifically with the host plasma membrane protein PCaP1 to participate in cell-to-cell movement.
      Title: Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement.
    8. MDP25 induces microtubule depolymerization.
      Title: MDP25, a novel calcium regulatory protein, mediates hypocotyl cell elongation by destabilizing cortical microtubules in Arabidopsis.
    9. PCaP1 has a high Cu(2+)-binding capacity with a relatively high affinity. PCaP1 lacks cysteine and histidine residues. A large number of glutamate residues may be involved in the Cu(2+) binding. [PCaP1 cation-binding protein]
      Title: A plasma membrane-associated protein of Arabidopsis thaliana AtPCaP1 binds copper ions and changes its higher order structure.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001036599.2
    NM_001036600.2
    NM_001203849.1
    NM_001203850.1
    NM_001341407.1
    NM_001341408.1
    NM_001341409.1
    NM_001341410.1
    NM_118145.4
    NM_179081.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables calcium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables calmodulin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables copper ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-3,4,5-trisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-3,4-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-3,5-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-4,5-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in N-terminal protein myristoylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to copper ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to iron ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to magnesium ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to magnesium starvation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to mannitol stimulus IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to molecule of bacterial origin IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to potassium ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to salt stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to sorbitol IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cortical microtubule organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cortical microtubule organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within intracellular transport of virus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of microtubule polymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of unidimensional cell growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of unidimensional cell growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of microtubule depolymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of microtubule depolymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to calcium ion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT involved_in response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in response to water deprivation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within stomatal closure IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasmic microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in plant-type vacuole HDA PubMed 
    located_in plasma membrane HDA PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasmodesma IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    plasma-membrane associated cation-binding protein 1
    NP_001031676.1
    • plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    NP_001031677.1
    • plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    NP_001190778.1
    • plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469).
    NP_001190779.1
    • plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469).
    NP_001320005.1
    • plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, chloroplast, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 6211 Blast hits to 3706 proteins in 536 species: Archae - 35; Bacteria - 949; Metazoa - 1704; Fungi - 697; Plants - 277; Viruses - 59; Other Eukaryotes - 2490 (source: NCBI BLink).
    NP_193759.1
    • plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, chloroplast, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 6213 Blast hits to 3707 proteins in 537 species: Archae - 35; Bacteria - 951; Metazoa - 1704; Fungi - 697; Plants - 277; Viruses - 59; Other Eukaryotes - 2490 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003075.7 Reference assembly

      Range
      10940414..10943561
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001341407.1 → NP_001320005.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429

    2. NM_001341408.1 → NP_001329494.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429

    3. NM_001341409.1 → NP_001329493.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429

    4. NM_001341410.1 → NP_001329492.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429

    5. NM_179081.5 → NP_849412.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_849412.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429
      Conserved Domains (1) summary
      pfam05558
      Location:1 → 225
      DREPP; DREPP plasma membrane polypeptide

    6. NM_001036600.2 → NP_001031677.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001031677.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429
      Conserved Domains (1) summary
      pfam05558
      Location:1 → 226
      DREPP; DREPP plasma membrane polypeptide

    7. NM_001036599.2 → NP_001031676.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001031676.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429
      Conserved Domains (1) summary
      pfam05558
      Location:1 → 225
      DREPP; DREPP plasma membrane polypeptide

    8. NM_001203849.1 → NP_001190778.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190778.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      F4JUT9
      Conserved Domains (1) summary
      pfam05558
      Location:1 → 143
      DREPP; DREPP plasma membrane polypeptide

    9. NM_001203850.1 → NP_001190779.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190779.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      F4JUT8
      Conserved Domains (1) summary
      pfam05558
      Location:1 → 166
      DREPP; DREPP plasma membrane polypeptide

    10. NM_118145.4 → NP_193759.1  plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_193759.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262
      UniProtKB/TrEMBL
      A0A5S9XU81, A0A654FR46, A0A7G2F429
      Conserved Domains (1) summary
      pfam05558
      Location:1 → 225
      DREPP; DREPP plasma membrane polypeptide
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96262.1

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