P5CS2 delta 1-pyrroline-5-carboxylate synthase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: P5CS2
Official Full Name: delta 1-pyrroline-5-carboxylate synthase 2
Primary source: TAIR:AT3G55610
Locus tag: AT3G55610
See related: Araport:AT3G55610
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: delta 1-pyrroline-5-carboxylate synthase 2
Summary: encodes delta 1-pyrroline-5-carboxylate synthetase B. Gene expression is induced by dehydration, high salt and ABA. Knock-out mutations in P5CS2 are embryo-lethal. P5CS2 appears to be present in different cells and/or different subcellular locations from P5CS1 in a tissue-dependent manner. Mutants are defective in pollen development.
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Genomic context

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See P5CS2 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 20

Sequence:: Chromosome: 3; NC_003074.8 (20624020..20629295, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cytosolic proline is required for basal freezing tolerance in Arabidopsis.
Title: Cytosolic proline is required for basal freezing tolerance in Arabidopsis.
P5CS1 and P5CS2 are expressed at similar levels and with the same pattern of expression in vegetative and floral shoot apical meristems as well as in axillary meristems.
Title: The proline biosynthetic genes P5CS1 and P5CS2 play overlapping roles in Arabidopsis flower transition but not in embryo development.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001125374.1

Supported by ESTs:

NM_115419.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables glutamate 5-kinase activity	IEA Inferred from Electronic Annotation more info
enables glutamate-5-semialdehyde dehydrogenase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-proline biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryo development ending in seed dormancy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hyperosmotic salinity response	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within pollen development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within proline biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within proline biosynthetic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to abscisic acid	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISM Inferred from Sequence Model more info
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: delta 1-pyrroline-5-carboxylate synthase 2

NP_001118846.1

delta 1-pyrroline-5-carboxylate synthase 2 (P5CS2); FUNCTIONS IN: oxidoreductase activity, catalytic activity, glutamate 5-kinase activity; INVOLVED IN: hyperosmotic salinity response, proline biosynthetic process, response to abscisic acid stimulus, embryo development ending in seed dormancy; LOCATED IN: chloroplast, cytoplasm; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, C globular stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Glutamate 5-kinase (InterPro:IPR001057), Glutamate 5-kinase, conserved site (InterPro:IPR019797), Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Aldehyde dehydrogenase, N-terminal (InterPro:IPR016162), Aldehyde dehydrogenase, C-terminal (InterPro:IPR016163), Delta l-pyrroline-5-carboxylate synthetase (InterPro:IPR005766), Aldehyde/histidinol dehydrogenase (InterPro:IPR016161), Aldehyde dehydrogenase (InterPro:IPR015590), Glutamate 5-kinase, ProB-related (InterPro:IPR005715); BEST Arabidopsis thaliana protein match is: delta1-pyrroline-5-carboxylate synthase 1 (TAIR:AT2G39800.4); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_191120.2

delta 1-pyrroline-5-carboxylate synthase 2 (P5CS2); FUNCTIONS IN: oxidoreductase activity, glutamate-5-semialdehyde dehydrogenase activity, catalytic activity, glutamate 5-kinase activity; INVOLVED IN: hyperosmotic salinity response, proline biosynthetic process, response to abscisic acid stimulus, embryo development ending in seed dormancy; LOCATED IN: chloroplast, cytoplasm; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, C globular stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Glutamate 5-kinase (InterPro:IPR001057), Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Glutamate 5-kinase, conserved site (InterPro:IPR019797), Aldehyde dehydrogenase, N-terminal (InterPro:IPR016162), Gamma-glutamyl phosphate reductase GPR, conserved site (InterPro:IPR020593), Aldehyde dehydrogenase, C-terminal (InterPro:IPR016163), Delta l-pyrroline-5-carboxylate synthetase (InterPro:IPR005766), Gamma-glutamyl phosphate reductase GPR (InterPro:IPR000965), Aldehyde/histidinol dehydrogenase (InterPro:IPR016161), Glutamate 5-kinase, ProB-related (InterPro:IPR005715); BEST Arabidopsis thaliana protein match is: delta1-pyrroline-5-carboxylate synthase 1 (TAIR:AT2G39800.4); Has 14836 Blast hits to 14828 proteins in 2349 species: Archae - 139; Bacteria - 9225; Metazoa - 151; Fungi - 264; Plants - 176; Viruses - 0; Other Eukaryotes - 4881 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

20624020..20629295 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_115419.5 → NP_191120.2 delta 1-pyrroline-5-carboxylate synthase 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_191120.2

Status: REVIEWED

UniProtKB/Swiss-Prot

P54888, Q9M061

UniProtKB/TrEMBL

A0A178VF78, A0A5S9XLT7, A0A7G2EY39

Conserved Domains (1) summary

PLN02418
Location:1 → 717

PLN02418; delta-1-pyrroline-5-carboxylate synthase
NM_001125374.1 → NP_001118846.1 delta 1-pyrroline-5-carboxylate synthase 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001118846.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A7G2EY39, B3H5E3

Conserved Domains (3) summary

cd07079
Location:296 → 621

ALDH_F18-19_ProA-GPR; Gamma-glutamyl phosphate reductase (GPR), aldehyde dehydrogenase families 18 and 19

PLN02418
Location:1 → 621

PLN02418; delta-1-pyrroline-5-carboxylate synthase

cl00452
Location:7 → 281

AAK; Amino Acid Kinases (AAK) superfamily, catalytic domain; present in such enzymes like N-acetylglutamate kinase (NAGK), carbamate kinase (CK), aspartokinase (AK), glutamate-5-kinase (G5K) and UMP kinase (UMPK). The AAK superfamily includes kinases that ...