NIT1 nitrilase 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: NIT1
Official Full Name: nitrilase 1
Primary source: TAIR:AT3G44310
Locus tag: AT3G44310
See related: Araport:AT3G44310
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: A. THALIANA NITRILASE 1; ATNIT1; NITI; nitrilase 1; NITRILE AMINOHYDROLASE
Summary: Mutants are resistant to indole-3-acetonitrile (IAN). NIT1 catalyzes the terminal activation step in indole-acetic acid biosynthesis. Predominantly expressed isoform of nitrilase isoenzyme family. Aggregation of NIT1 in cells directly abutting wound sites is one of the earliest events associated with wound and herbicide-induced cell death. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide. It is also involved in the conversion of IAN to IAM (indole-3-acetamide) and other non-auxin-related metabolic processes.
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Genomic context

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See NIT1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 7

Sequence:: Chromosome: 3; NC_003074.8 (15986624..15989505)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The metabolite repair enzyme Nit1 is a dual-targeted amidase that disposes of damaged glutathione in Arabidopsis.
Title: The metabolite repair enzyme Nit1 is a dual-targeted amidase that disposes of damaged glutathione in Arabidopsis.
NIT1 regulates the exit from proliferation, genome stability and plant development.
Title: NITRILASE1 regulates the exit from proliferation, genome stability and plant development.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MASC_STS16098 (e-PCR)
- UniSTS:258848

Homology

Orthologs from OrthoDB

NM_001084765.1

Supported by ESTs:

NM_001339127.1

Supported by ESTs:

NM_001339128.1

Supported by ESTs:

NM_180680.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables cyanoalanine nitrilase activity	IEA Inferred from Electronic Annotation more info
enables indole-3-acetonitrile nitrilase activity	IDA Inferred from Direct Assay more info	PubMed
enables indole-3-acetonitrile nitrile hydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitrilase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within indoleacetic acid biosynthetic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in apoplast	HDA more info	PubMed
located_in chloroplast	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in plasma membrane	ISM Inferred from Sequence Model more info
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: nitrilase 1

NP_001078234.1

nitrilase 1 (NIT1); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: indoleacetic acid biosynthetic process; LOCATED IN: apoplast, chloroplast, plasma membrane; EXPRESSED IN: guard cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 2 (TAIR:AT3G44300.1); Has 7572 Blast hits to 7496 proteins in 1731 species: Archae - 139; Bacteria - 4902; Metazoa - 443; Fungi - 562; Plants - 319; Viruses - 11; Other Eukaryotes - 1196 (source: NCBI BLink).

NP_001319680.1

nitrilase 1 (NIT1); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: indoleacetic acid biosynthetic process; LOCATED IN: apoplast, chloroplast, plasma membrane; EXPRESSED IN: guard cell, cultured cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 2 (TAIR:AT3G44300.1); Has 4645 Blast hits to 4615 proteins in 1269 species: Archae - 39; Bacteria - 2832; Metazoa - 304; Fungi - 427; Plants - 285; Viruses - 0; Other Eukaryotes - 758 (source: NCBI BLink).

NP_851011.1

nitrilase 1 (NIT1); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: indoleacetic acid biosynthetic process; LOCATED IN: apoplast, chloroplast, plasma membrane; EXPRESSED IN: guard cell, cultured cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 2 (TAIR:AT3G44300.1); Has 7572 Blast hits to 7496 proteins in 1731 species: Archae - 139; Bacteria - 4902; Metazoa - 443; Fungi - 562; Plants - 319; Viruses - 11; Other Eukaryotes - 1196 (source: NCBI BLink).