PME3 pectin methylesterase 3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PME3
Official Full Name: pectin methylesterase 3
Primary source: TAIR:AT3G14310
Locus tag: AT3G14310
See related: Araport:AT3G14310
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATPME3; OVERLY ZINC SENSITIVE 2; OZS2; pectin methylesterase 3
Summary: encodes a pectin methylesterase, targeted by a cellulose binding protein (CBP) from the parasitic nematode Heterodera schachtii during parasitism.
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Genomic context

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See PME3 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 3

Sequence:: Chromosome: 3; NC_003074.8 (4771833..4775258, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Analysis of the ability of Glu-68AtPMEI7 and Glu-75AtPMEI7 to establish hydrogen bonds with AtPME3 revealed that these residues may play an important role in defining complex stability and formation.
Title: Structural and dynamical characterization of the pH-dependence of the pectin methylesterase-pectin methylesterase inhibitor complex.
alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings
Title: AtPME3, a ubiquitous cell wall pectin methylesterase of Arabidopsis thaliana, alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings.
Docking analysis indicated that the inhibition of AtPME3 could occur via the interaction of AtPMEI7 with a PME ligand-binding cleft structure.
Title: Tuning of Pectin Methylesterification: PECTIN METHYLESTERASE INHIBITOR 7 MODULATES THE PROCESSIVE ACTIVITY OF CO-EXPRESSED PECTIN METHYLESTERASE 3 IN A pH-DEPENDENT MANNER.
Transgenic lines overexpressing the DUF642 proteins encoded by At4g32460 and At5g11420 showed increased pectin methyl esterase 3 activity during seed germination, and improved seed germination performance.
Title: BIIDXI, the At4g32460 DUF642 gene, is involved in pectin methyl esterase regulation during Arabidopsis thaliana seed germination and plant development.
role of pectin methyl esterase and preference in wall-associated kinase-activated, EDS1/PAD4-dependent stress response in Arabidopsis
Title: Requirement for pectin methyl esterase and preference for fragmented over native pectins for wall-associated kinase-activated, EDS1/PAD4-dependent stress response in Arabidopsis.
Ectopic expression of AtPME3 causes strong Zn(2+) hypersensitivity that is tightly correlated with transcript abundance. Together these observations suggest detrimental effects on Golgi-localized processes.
Title: A mutation in the Arabidopsis thaliana cell wall biosynthesis gene pectin methylesterase 3 as well as its aberrant expression cause hypersensitivity specifically to Zn.
The biochemical characterization of the Arabidopsis thaliana pectin methylesterase 3 gene (AtPME3; At3g14310) and its role in plant development, is reported.[PME3]
Title: Identification of pectin methylesterase 3 as a basic pectin methylesterase isoform involved in adventitious rooting in Arabidopsis thaliana.
Upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of AtPME3 that acts as a susceptibility factor and is required for the initial colonization of the host tissue.
Title: Pectin methylesterase is induced in Arabidopsis upon infection and is necessary for a successful colonization by necrotrophic pathogens.
PME3 directly interacts with the nematode cellulose binding protein which aids cyst nematode parasitism.
Title: Cellulose binding protein from the parasitic nematode Heterodera schachtii interacts with Arabidopsis pectin methylesterase: cooperative cell wall modification during parasitism.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_112289.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables enzyme inhibitor activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables pectinesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables pectinesterase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables pectinesterase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell wall modification	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_negative_effect defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_negative_effect defense response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of seed germination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pectin catabolic process	IEA Inferred from Electronic Annotation more info
involved_in pectin catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of unidimensional cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to fungus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to fungus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to nematode	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to zinc ion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in root development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_positive_effect root hair cell development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apoplast	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in plant-type cell wall	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: pectin methylesterase 3

NP_188048.1

pectin methylesterase 3 (PME3); FUNCTIONS IN: pectinesterase activity; INVOLVED IN: response to nematode; LOCATED IN: cell wall, apoplast, plasma membrane, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Pectinesterase, active site (InterPro:IPR018040), Pectin lyase fold/virulence factor (InterPro:IPR011050), Pectinesterase, catalytic (InterPro:IPR000070), Pectinesterase inhibitor (InterPro:IPR006501), Pectin lyase fold (InterPro:IPR012334); BEST Arabidopsis thaliana protein match is: pectin methylesterase 2 (TAIR:AT1G53830.1); Has 3052 Blast hits to 2982 proteins in 347 species: Archae - 6; Bacteria - 639; Metazoa - 1; Fungi - 199; Plants - 2181; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

4771833..4775258 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_112289.3 → NP_188048.1 pectin methylesterase 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

O49006, Q56X61, Q93YZ2, Q9LUL7

UniProtKB/TrEMBL

A0A178VDL9, A0A5S9XCE0, A0A654F735

Conserved Domains (1) summary

PLN02313
Location:1 → 592

PLN02313; Pectinesterase/pectinesterase inhibitor