CKI1 Signal transduction histidine kinase [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: CKI1
Official Full Name: Signal transduction histidine kinase
Primary source: TAIR:AT2G47430
Locus tag: AT2G47430
See related: Araport:AT2G47430
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: CYTOKININ-INDEPENDENT 1; HISTIDINE KINASE; T30B22.27
Summary: Encodes a putative plasma membrane-bound hybrid histidine kinase and cytokinin sensor that is expressed within the female gametophyte.
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Genomic context

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See CKI1 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 10

Sequence:: Chromosome: 2; NC_003071.7 (19459045..19464087, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that the light-dependent regulation of CYTOKININ-INDEPENDENT 1 protein (CKI1) provides a plausible mechanistic link underlying the well-known interaction between light- and cytokinin-controlled plant development.
Title: Light Controls Cytokinin Signaling via Transcriptional Regulation of Constitutively Active Sensor Histidine Kinase CKI1.
AHP2, AHP3, and AHP5 are the major factors acting downstream of CKI1 in the two-component cytokinin signaling pathway to promote Arabidopsis female gametophyte development.
Title: AHP2, AHP3, and AHP5 act downstream of CKI1 in Arabidopsis female gametophyte development.
The functional relevance of the conformational dynamics observed in the beta3-alpha3 loop of CKI1RD was supported by a comparison with another A. thaliana histidine kinase, ETR1. In contrast to the highly dynamic beta3-alpha3 loop of CKI1RD, the corresponding loop of the ETR1 receiver domain (ETR1RD) exhibited little conformational exchange and adopted a different orientation in crystals.
Title: Conformational dynamics are a key factor in signaling mediated by the receiver domain of a sensor histidine kinase from Arabidopsis thaliana.
Studied sequence dependence of (13) C and (15) N chemical shifts in the receiver domain of CKI1 protein from Arabidopsis thaliana, CKI1RD , and its complexed form, CKI1RD *Mg(2+), using MD/DFT calculations. MD simulations.
Title: The influence of Mg2+ coordination on 13C and 15N chemical shifts in CKI1RD protein domain from experiment and molecular dynamics/density functional theory calculations.
CKI1-directed specification of the endosperm precursor central cell results in seeds containing an embryo and an endosperm.
Title: The CKI1 Histidine Kinase Specifies the Female Gametic Precursor of the Endosperm.
The C-terminal receiver domain of ne kinase CKI1 (CKI1(RD) ) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities.
Title: Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.
CKI1-activated signaling is dependent on AHP genes which is essential for female gametophyte development. CKI1 also plays an important role during vegetative growth by regulating cell expansion.
Title: Arabidopsis histidine kinase CKI1 acts upstream of histidine phosphotransfer proteins to regulate female gametophyte development and vegetative growth.
The results indicate that the cytokinin-independent activity of CKI1 and cytokinin-induced AHK2 and AHK3 are important for vascular bundle formation in Arabidopsis.
Title: The histidine kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 regulate vascular tissue development in Arabidopsis shoots.
Crystals of the recombinant RD of the Arabidopsis thaliana HK CYTOKININ-INDEPENDENT1 (CKI1(RD)) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and glycerol as a cryoprotectant
Title: Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana.
CKI-1 is involved in cytokinin perception and demonstrates the existence of a feedback loop altering cytokinin metabolism in response to the level of receptor abundance
Title: CYTOKININ INDEPENDENT-1 regulates levels of different forms of cytokinin in Arabidopsis and mediates response to nutrient stress.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

At2g47430_Arabidopsis_lyrata_ssp._lyrata_CAN10 (e-PCR)
- UniSTS:510290

At2g47430_Arabidopsis_lyrata_ssp._petraea_GER10 (e-PCR)
- UniSTS:510290

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables phosphorelay response regulator activity	TAS Traceable Author Statement more info	PubMed
enables phosphorelay sensor kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein histidine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein histidine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cytokinin-activated signaling pathway	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within embryo sac development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phloem or xylem histogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
involved_in secondary growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	ISM Inferred from Sequence Model more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: Signal transduction histidine kinase

NP_182265.1

CYTOKININ-INDEPENDENT 1 (CKI1); CONTAINS InterPro DOMAIN/s: Signal transduction histidine kinase, homodimeric (InterPro:IPR009082), CheY-like (InterPro:IPR011006), Signal transduction histidine kinase, core (InterPro:IPR005467), Signal transduction response regulator, receiver domain (InterPro:IPR001789), Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain (InterPro:IPR003661), ATPase-like, ATP-binding domain (InterPro:IPR003594), Signal transduction histidine kinase-related protein, C-terminal (InterPro:IPR004358); BEST Arabidopsis thaliana protein match is: histidine kinase 1 (TAIR:AT2G17820.1); Has 89821 Blast hits to 76986 proteins in 2917 species: Archae - 765; Bacteria - 78909; Metazoa - 25; Fungi - 2111; Plants - 1771; Viruses - 21; Other Eukaryotes - 6219 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

19459045..19464087 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_130311.2 → NP_182265.1 Signal transduction histidine kinase [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_182265.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A5YY37, O22267

UniProtKB/TrEMBL

A0A178VSB0, A0A5S9X7R6

Conserved Domains (4) summary

smart00387
Location:511 → 665

HATPase_c; Histidine kinase-like ATPases

smart00388
Location:396 → 461

HisKA; His Kinase A (phosphoacceptor) domain

PRK11107
Location:367 → 1114

PRK11107; hybrid sensory histidine kinase BarA; Provisional

cd00156
Location:989 → 1117

REC; Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems; ...