serp serpentine [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: serpprovided by FlyBase
Official Full Name: serpentineprovided by FlyBase
Primary source: FLYBASE:FBgn0260653
Locus tag: Dmel_CG32209
See related: AllianceGenome:FB:FBgn0260653
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG32209; CG8747; CG8748; DmCDA1; Dmel\CG32209; l(3)76BDk; Serp
Summary: Enables chitin binding activity and chitin deacetylase activity. Involved in chitin metabolic process; chitin-based cuticle development; and regulation of tube length, open tracheal system. Located in several cellular components, including cell surface; chitin-based extracellular matrix; and late endosome. Is expressed in several structures, including embryonic foregut; embryonic head epidermis; embryonic salivary gland duct; embryonic ventral epidermis; and epithelium. [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 76C2-76C2; 3-46 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (19700977..19708015, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (19694077..19701115, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DmCDA1 (Serpentine, Serp) and DmCDA2 (Vermiform, Verm) have distinct functions during Drosophila adult wing cuticle differentiation. Chitosan staining revealed that Serp is the major enzyme responsible for chitin deacetylation during wing cuticle formation, while Verm does not seem to be needed for this process. Indeed, it is questionable whether Verm is a chitin deacetylase at all.
Title: The putative chitin deacetylases Serpentine and Vermiform have non-redundant functions during Drosophila wing development.
we find that EGFR regulates the apical determinant Crb and the extracellular matrix regulator Serp, two factors previously known to control tube length. EGFR regulates the organisation of endosomes in which Crb and Serp proteins are loaded
Title: EGFR controls Drosophila tracheal tube elongation by intracellular trafficking regulation.
An endosomal-retrieval machinery consisting of Rab9, retromer and actin nucleator WASH (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) regulates selective recycling of the luminal protein Serpentine in the Drosophila trachea.
Title: Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control.
Serpentine (serp) and Vermiform (verm) encode similar proteins with an LDL-receptor ligand binding motif and chitin binding and deacetylation domains and are required for the normal fibrillar structure of chitin.
Title: serpentine and vermiform encode matrix proteins with chitin binding and deacetylation domains that limit tracheal tube length in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables chitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chitin binding	IEA Inferred from Electronic Annotation more info
enables chitin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables chitin deacetylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables chitin deacetylase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in adult chitin-based cuticle development	IBA Inferred from Biological aspect of Ancestor more info
involved_in adult chitin-based cuticle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in chitin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chitin-based cuticle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in larval chitin-based cuticle development	IBA Inferred from Biological aspect of Ancestor more info
involved_in larval chitin-based cuticle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in open tracheal system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of tube length, open tracheal system	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of tube length, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in basement membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in chitin-based extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serpentine

Names: CG32209-PB; CG32209-PC; l(3)76BDk; serp-PB; serp-PC

NP_001262062.1

EC 3.5.-.-

EC 3.5.1.41

NP_730444.1

EC 3.5.-.-

EC 3.5.1.41

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

19700977..19708015 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_168811.2 → NP_730444.1 serpentine, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730444.1

Status: REVIEWED

UniProtKB/TrEMBL

Q86P23, Q9VW34

Related

FBpp0074721

Conserved Domains (3) summary

cd10974
Location:200 → 475

CE4_CDA_like_1; Putative catalytic domain of chitin deacetylase-like proteins with additional chitin-binding peritrophin-A domain (ChBD) and/or a low-density lipoprotein receptor class A domain (LDLa)

cd00112
Location:125 → 159

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...

pfam01607
Location:48 → 105

CBM_14; Chitin binding Peritrophin-A domain
NM_001275133.1 → NP_001262062.1 serpentine, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262062.1

Status: REVIEWED

UniProtKB/TrEMBL

Q86P23, Q9VW34

Conserved Domains (3) summary

cd10974
Location:200 → 475

CE4_CDA_like_1; Putative catalytic domain of chitin deacetylase-like proteins with additional chitin-binding peritrophin-A domain (ChBD) and/or a low-density lipoprotein receptor class A domain (LDLa)

cd00112
Location:125 → 159

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...

pfam01607
Location:48 → 105

CBM_14; Chitin binding Peritrophin-A domain