Nrx-IV Neurexin IV [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Nrx-IVprovided by FlyBase
Official Full Name: Neurexin IVprovided by FlyBase
Primary source: FLYBASE:FBgn0013997
Locus tag: Dmel_CG6827
See related: AllianceGenome:FB:FBgn0013997
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG682; CG6827; CNTNAP2; CT21123; Dmel\CG6827; Dnrx; l(3)68Ff; l(3)rsg23; neurexin; nrx; Nrx; NRX; nrx IV; Nrx IV; NRX IV; nrx-iv; nrx-IV; nrx4; Nrx4; nrxIV; NrxIV; Nx IV; rsg23
Summary: Involved in several processes, including cell adhesion involved in heart morphogenesis; nervous system development; and terminal button organization. Located in plasma membrane; pleated septate junction; and presynaptic active zone. Is expressed in several structures, including glial cell; gut section; internal sense organ; peripheral nervous system; and sensory organ cell. Used to study autism spectrum disorder and autosomal recessive intellectual developmental disorder. Human ortholog(s) of this gene implicated in several diseases, including Pitt-Hopkins syndrome; autism spectrum disorder (multiple); communication disorder (multiple); cortical dysplasia-focal epilepsy syndrome; and social phobia. Orthologous to several human genes including CNTNAP2 (contactin associated protein 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 68F6-68F7; 3-38 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (12146519..12156990)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (12139619..12150090)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Screen for novel regulators of synapse morphogenesis at the larval neuromuscular junction in Drosophila identified miR-34 as conserved synapse-regulatory miRNA and junctional receptor CNTNAP4/Neurexin-IV (Nrx-IV) and the membrane cytoskeletal effector Adducin/Hu-li tai shao (Hts) as proteins whose synaptic expression is restricted by miR-34.
Title: The conserved microRNA miR-34 regulates synaptogenesis via coordination of distinct mechanisms in presynaptic and postsynaptic cells.
DNrx promotes ephrin clustering and reveals that ephrin/Eph signaling from adjacent L4 neurons restricts axonal branches of L4 neurons in columns.
Title: Neurexin Restricts Axonal Branching in Columns by Promoting Ephrin Clustering.
Daughterless and Tcf4 are expressed in postmitotic neurons in Drosophila melanogaster and mice, respectively, where they function to restrict neurite branching and synapse formation in part by restricting the expression of the cell adhesion molecule Neurexin.[Tcf4]
Title: Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.
Neurexin mediates retinoid transport and subsequent rhodopsin maturation in visual function; the Drosophila homolog of alpha-neurexin is critical for fly visual function.
Title: Neurexin regulates visual function via mediating retinoid transport to promote rhodopsin maturation.
The regulation of glial-specific splicing of Neurexin IV requires HOW and Cdk12 activity.
Title: The regulation of glial-specific splicing of Neurexin IV requires HOW and Cdk12 activity.
The first Laminin G-EGF-Laminin G module in the extracellular region of Neurexin IV is necessary for the localization of and association with Contactin.
Title: A Laminin G-EGF-Laminin G module in Neurexin IV is essential for the apico-lateral localization of Contactin and organization of septate junctions.
Cno function as a novel regulator of neuron-glia interactions, forming a complex with Shg, Wrapper and Neurexin IV.
Title: Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
The possibility that neurexin is present postsynaptically raises new hypotheses regarding neurexin function in synapses, and our results provide new insights into the role of neurexin in synapse development
Title: Neurexin in embryonic Drosophila neuromuscular junctions.
Nrx IV is essential for proper Robo localization and repulsive axon guidance, and is a novel interacting partner of the Slit/Robo signaling pathway.
Title: Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
Although both Neurexin IV isoforms are able to bind Wrapper, the neuronal isoform has a higher affinity for Wrapper
Title: Drosophila Neurexin IV stabilizes neuron-glia interactions at the CNS midline by binding to Wrapper.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Process	Evidence Code	Pubs
involved_in axon ensheathment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell adhesion involved in heart morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-cell junction organization	TAS Traceable Author Statement more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of glial blood-brain barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nerve maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in presynaptic membrane assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	TAS Traceable Author Statement more info	PubMed
involved_in regulation of tube size, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septate junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic target recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in terminal button organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in pleated septate junction	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neurexin IV

Names: CG6827-PA; CG6827-PB; Neurexin-4; Neurexin-IV; Nrx-IV-PA; Nrx-IV-PB; neurexin; neurexin 4; neurexinIV; rose-gespleten region interval 23

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

12146519..12156990

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_168491.3 → NP_729787.2 neurexin IV, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_729787.2

Status: REVIEWED

UniProtKB/TrEMBL

Q6NN26, Q8IQH0

Conserved Domains (7) summary

smart00294
Location:1239 → 1255

4.1m; putative band 4.1 homologues' binding motif

smart00231
Location:46 → 185

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain

cd00054
Location:966 → 998

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00057
Location:57 → 184

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain; Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes.

cd00110
Location:192 → 340

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:546 → 575

EGF; EGF-like domain

pfam02210
Location:824 → 943

Laminin_G_2; Laminin G domain
NM_079310.3 → NP_524034.2 neurexin IV, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524034.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94887, Q9VTU5

UniProtKB/TrEMBL

Q6NN26

Related

FBpp0075730

Conserved Domains (7) summary

smart00294
Location:1239 → 1255

4.1m; putative band 4.1 homologues' binding motif

smart00231
Location:46 → 185

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain

cd00054
Location:966 → 998

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00057
Location:57 → 184

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain; Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes.

cd00110
Location:192 → 340

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:546 → 575

EGF; EGF-like domain

pfam02210
Location:824 → 943

Laminin_G_2; Laminin G domain