Slc1a5 solute carrier family 1 member 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc1a5provided by RGD
Official Full Name: solute carrier family 1 member 5provided by RGD
Primary source: RGD:708512
See related: EnsemblRapid:ENSRNOG00000015948 AllianceGenome:RGD:708512
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Asct2; ATB(0); Slc1a7; H4-ASCT2
Summary: Enables L-amino acid transmembrane transporter activity; antiporter activity; and ligand-gated channel activity. Involved in carboxylic acid transport. Predicted to be located in basal plasma membrane. Predicted to be active in plasma membrane. Orthologous to human SLC1A5 (solute carrier family 1 member 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Thymus (RPKM 228.8), Spleen (RPKM 165.3) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q21

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (86584949..86599052)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (77456849..77470952)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (78710686..78724789)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interaction of the neutral amino acid transporter ASCT2 with basic amino acids.
Title: Interaction of the neutral amino acid transporter ASCT2 with basic amino acids.
the selectivity profile of D-serine uptake in cultured rat hippocampal astrocytes is consistent with uptake mediated by both ASCT1 and ASCT2. Together these data suggest that SLC1A4 (ASCT1) may represent an important route of Na-dependent D-serine flux in the brain that has the ability to regulate extracellular D-serine and thereby NMDA receptor activity.
Title: D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.
Hypoxic exposure significantly increased SLC1A5 expression in neurovascular unit cells in vitro, but suppressed EAAT2 expression.
Title: Expression of Glutamate and Glutamine Transporter Proteins in Neurovascular Unit Cells In Vitro.
D-serine heteroexchange through ASC transporters, in particular low affinity ASCT2, is present in vivo and may constitute a key component in the regulation of D-serine extracellular concentration.
Title: In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors.
Basic amino acid transporter is localized in a punctuate manner following phorbol myristate acetate treatment when more pronounced bigger puncta are visible, indicating formation of bigger plasma membrane microdomains or astrocyte intracellular vesicles.
Title: Regulation of amino acid/carnitine transporter B 0,+ (ATB 0,+) in astrocytes by protein kinase C: independent effects on raft and non-raft transporter subpopulations.
The data demonstrated that the inhibition of ASCT2 by mercury compounds was due to covalent reactions with Cys residue(s) of the transporter.
Title: Inactivation by Hg2+ and methylmercury of the glutamine/amino acid transporter (ASCT2) reconstituted in liposomes: Prediction of the involvement of a CXXC motif by homology modelling.
Leptin controls active glutamine entry through reduction of both B(0)AT1 and ASCT2 protein trafficking to the apical plasma membrane and modulation of their gene expression.
Title: Luminal leptin inhibits L-glutamine transport in rat small intestine: involvement of ASCT2 and B0AT1.
ASCT2 accepts also glutamate as substrate and the membrane potential did not affect the glutamine/glutamine antiport whereas it stimulated about two fold the glutamine/glutamate antiport rate.
Title: The glutamine/amino acid transporter (ASCT2) reconstituted in liposomes: electrical nature of the glutamine/glutamate antiport.
the activity and expression of the ASCT2 transporter were considerably lower in the spontaneously hypertensive rats
Title: Underexpression of the Na+-dependent neutral amino acid transporter ASCT2 in the spontaneously hypertensive rat kidney.
The reconstituted glutamine/amino acid transporter functionally corresponds to the Asct2 protein.
Title: Reconstitution into liposomes of the glutamine/amino acid transporter from renal cell plasma membrane: functional characterization, kinetics and activation by nucleotides.

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General gene information

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Markers

AU048745 (e-PCR)
- UniSTS:145195

D1Wox77 (e-PCR)
- UniSTS:518999

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables L-amino acid transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables L-aspartate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-aspartate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables L-aspartate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables L-glutamine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables L-serine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables acidic amino acid transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables antiporter activity	IEA Inferred from Electronic Annotation more info
enables antiporter activity	ISO Inferred from Sequence Orthology more info
enables ligand-gated channel activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables neutral L-amino acid transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables neutral L-amino acid transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables symporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-alpha-amino acid transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in L-aspartate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in L-aspartate import across plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in L-aspartate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in L-glutamine import across plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in L-serine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in acidic amino acid transport	IEA Inferred from Electronic Annotation more info
involved_in dicarboxylic acid transport	IEA Inferred from Electronic Annotation more info
involved_in erythrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in glutamine secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in glutamine transport	ISO Inferred from Sequence Orthology more info
involved_in neutral amino acid transport	IDA Inferred from Direct Assay more info	PubMed
involved_in neutral amino acid transport	ISO Inferred from Sequence Orthology more info
involved_in protein homotrimerization	ISO Inferred from Sequence Orthology more info
involved_in transport across blood-brain barrier	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
located_in melanosome	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neutral amino acid transporter B(0)

Names: ASC-like Na(+)-dependent neutral amino acid transporter ASCT2; CAZ-associated structural protein; H4-system ASC-like transporter; insulin-activated amino acid transporter; sodium-dependent neutral amino acid transporter ASCT2; sodium-dependent neutral amino acid transporter type 2; solute carrier family 1 (neutral amino acid transporter), member 5; solute carrier family 1, member 7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_175758.3 → NP_786934.1 neutral amino acid transporter B(0)

See identical proteins and their annotated locations for NP_786934.1

Status: PROVISIONAL

Source sequence(s)

BC080242

UniProtKB/Swiss-Prot

D3ZJ25

UniProtKB/TrEMBL

F7F8V1, Q9Z1J7

Related

ENSRNOP00000021455.3, ENSRNOT00000021455.8

Conserved Domains (1) summary

pfam00375
Location:87 → 480

SDF; Sodium:dicarboxylate symporter family

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

86584949..86599052

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039103908.2 → XP_038959836.1 neutral amino acid transporter B(0) isoform X1

Conserved Domains (1) summary

pfam00375
Location:38 → 290

SDF; Sodium:dicarboxylate symporter family