spas-1 Spastin homolog [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: spas-1
Official Full Name: Spastin homolog
Primary source: WormBase:WBGene00016045
Locus tag: CELE_C24B5.2
See related: AllianceGenome:WB:WBGene00016045
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables several functions, including ATP binding activity; ATP hydrolysis activity; and microtubule severing ATPase activity. Involved in several processes, including microtubule cytoskeleton organization; positive regulation of microtubule depolymerization; and vulval development. Located in microtubule cytoskeleton and perinuclear region of cytoplasm. Part of protein-containing complex. Is expressed in several structures, including intestine; mechanosensory neurons; somatic nervous system; tail ganglion; and vulva. Used to study hereditary spastic paraplegia. Human ortholog(s) of this gene implicated in hereditary spastic paraplegia 4. Orthologous to human SPAST (spastin). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See spas-1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 7

Sequence:: Chromosome: V; NC_003283.11 (9175789..9177997)

Chromosome V - NC_003283.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003283.11

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The C-terminal alpha-helix of SPAS-1, a Caenorhabditis elegans spastin homologue, is crucial for microtubule severing.
Title: The C-terminal α-helix of SPAS-1, a Caenorhabditis elegans spastin homologue, is crucial for microtubule severing.
We propose that microtubule binding domain (MTBD) of SPAS-1 plays a critical role in enrichment of SPAS-1 to microtubules, where SPAS-1 is concentrated and able to form a stable hexamer, subsequently its ATPase activity is stimulated.
Title: Conserved aromatic and basic amino acid residues in the pore region of Caenorhabditis elegans spastin play critical roles in microtubule severing.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C24B5.2 (e-PCR)
- UniSTS:312183

C24B5.5 (e-PCR)
- UniSTS:317656

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	NAS Non-traceable Author Statement more info	PubMed
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	NAS Non-traceable Author Statement more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule severing ATPase activity	IC Inferred by Curator more info	PubMed
enables microtubule severing ATPase activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule severing ATPase activity	IEA Inferred from Electronic Annotation more info
enables microtubule severing ATPase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in microtubule bundle formation	NAS Non-traceable Author Statement more info	PubMed
involved_in microtubule cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule severing	IDA Inferred from Direct Assay more info	PubMed
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in nucleus localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in vulval development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: Spastin homolog

NP_001256115.1

Confirmed by transcript evidence

NP_741586.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003283.11 Reference assembly

Range

9175789..9177997

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001269186.3 → NP_001256115.1 Spastin homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001256115.1

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EEF8, Q7M3K5, Q8MNU9, Q8MNV0

Conserved Domains (2) summary

COG0464
Location:209 → 498

SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]

PRK05703
Location:105 → 298

flhF; flagellar biosynthesis protein FlhF
NM_171501.7 → NP_741586.1 Spastin homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741586.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8MNV0

Conserved Domains (4) summary

COG0464
Location:1 → 437

SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]

pfam00004
Location:214 → 344

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:406 → 450

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:177 → 233

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases