Abstract
Four genes of Escherichia coli whose products are needed to reduce biotin-d-sulfoxide to biotin have been mapped: bisA next to chlA, bisB next to chlE, bisC linked to xyl, and bisD next to chlG. A defective lambda transducing phage, lambdadbis5, which carries all the bacterial genes between the lambda attachment site and chlE, was isolated and shown to have lost the phage genes from int through Q.
MeSH terms
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Biotin / biosynthesis
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Biotin / metabolism*
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Chromosome Mapping
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Coliphages
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DNA Viruses
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Escherichia coli / enzymology
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Escherichia coli / growth & development
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Escherichia coli / metabolism*
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Genes*
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Genetic Complementation Test
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Hot Temperature
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Lysogeny
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Methionine / biosynthesis
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Mitomycins / pharmacology
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Mutagens
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Mutation*
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Nitrate Reductases / metabolism
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Nitrosoguanidines
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Oxidation-Reduction
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Oxidoreductases / metabolism
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Sulfoxides / metabolism*
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Transduction, Genetic
Substances
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Mitomycins
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Mutagens
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Nitrosoguanidines
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Sulfoxides
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Biotin
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Methionine
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Oxidoreductases
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Nitrate Reductases