AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene

FEBS Lett. 2001 Jul 6;500(3):177-82. doi: 10.1016/s0014-5793(01)02599-6.

Abstract

SNAREs are membrane-associated proteins that play a central role in vesicle targeting and intra-cellular membrane fusion reactions in eukaryotic cells. Here we describe the identification of AtBS14a and AtBS14b, putative SNAREs from Arabidopsis thaliana that share 60% amino acid sequence identity. Both AtBS14a and BS14b are dosage suppressors of the temperature-sensitive growth defect in sft1-1 cells and over-expression of either AtBS14a or AtBS14b can support the growth of sft1Delta cells but not bet1Delta cells. These data together with structure-function and biochemical studies presented herein suggest that AtBS14a and AtBS14b share properties that are consistent with them being members of the Bet1/Sft1 SNARE protein family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins*
  • Cell Division / physiology
  • Genetic Complementation Test
  • Golgi Apparatus / metabolism
  • Intracellular Membranes / metabolism
  • Intracellular Membranes / ultrastructure
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Mutation
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Qc-SNARE Proteins
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • SNARE Proteins
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Temperature
  • Transformation, Genetic
  • Vesicular Transport Proteins*

Substances

  • Arabidopsis Proteins
  • At4g14455 protein, Arabidopsis
  • AtBS14a protein, Arabidopsis
  • BET1 protein, Zea mays
  • Membrane Proteins
  • Plant Proteins
  • Qc-SNARE Proteins
  • Recombinant Fusion Proteins
  • SFT1 protein, S cerevisiae
  • SNARE Proteins
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins

Associated data

  • GENBANK/AF368175
  • GENBANK/AY033334