Abstract
SNAREs are membrane-associated proteins that play a central role in vesicle targeting and intra-cellular membrane fusion reactions in eukaryotic cells. Here we describe the identification of AtBS14a and AtBS14b, putative SNAREs from Arabidopsis thaliana that share 60% amino acid sequence identity. Both AtBS14a and BS14b are dosage suppressors of the temperature-sensitive growth defect in sft1-1 cells and over-expression of either AtBS14a or AtBS14b can support the growth of sft1Delta cells but not bet1Delta cells. These data together with structure-function and biochemical studies presented herein suggest that AtBS14a and AtBS14b share properties that are consistent with them being members of the Bet1/Sft1 SNARE protein family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis Proteins*
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Cell Division / physiology
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Genetic Complementation Test
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Golgi Apparatus / metabolism
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Intracellular Membranes / metabolism
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Intracellular Membranes / ultrastructure
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Multigene Family
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Mutation
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Qc-SNARE Proteins
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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SNARE Proteins
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Temperature
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Transformation, Genetic
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Vesicular Transport Proteins*
Substances
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Arabidopsis Proteins
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At4g14455 protein, Arabidopsis
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AtBS14a protein, Arabidopsis
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BET1 protein, Zea mays
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Membrane Proteins
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Plant Proteins
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Qc-SNARE Proteins
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Recombinant Fusion Proteins
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SFT1 protein, S cerevisiae
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SNARE Proteins
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Saccharomyces cerevisiae Proteins
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Vesicular Transport Proteins
Associated data
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GENBANK/AF368175
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GENBANK/AY033334