Protein Family Models

The endonuclease MutS2 resembles the DNA mismatch repair enzyme MutS1, but instead acts in the suppression of homologous recombination.

Date:

2024-11-07

This family includes the Smr (Small MutS Related) proteins, and the C-terminal region of the MutS2 protein. It has been suggested that this domain interacts with the MutS1 Swiss:P23909 protein in the case of Smr proteins and with the N-terminal MutS related region of MutS2 Swiss:P94545 [1]. This domain exhibits nicking endonuclease activity that might have a role in mismatch repair or genetic recombination. It shows no significant double strand cleavage or exonuclease activity [2]. The full-length Swiss:Q86UW6 also has the polynucleotide kinase activity. [1]. 10431172. Smr: a bacterial and eukaryotic homologue of the C-terminal region of the MutS2 family. Moreira D, Philippe H;. Trends Biochem Sci 1999;24:298-300. [2]. 12730195. Identification and characterization of BCL-3-binding protein: implications for transcription and DNA repair or recombination. Watanabe N, Wachi S, Fujita T;. J Biol Chem. 2003;278:26102-26110. (from Pfam)

Date:

2024-10-16

This domain is found in proteins of the MutS family (DNA mismatch repair proteins) and is found associated with Pfam:PF01624, Pfam:PF05188, Pfam:PF05192 and Pfam:PF05190. The mutS family of proteins is named after the Salmonella typhimurium MutS protein involved in mismatch repair; other members of the family included the eukaryotic MSH 1,2,3, 4,5 and 6 proteins. These have various roles in DNA repair and recombination. Human MSH has been implicated in non-polyposis colorectal carcinoma (HNPCC) and is a mismatch binding protein [2]. The aligned region corresponds with domain V of Thermus aquaticus MutS as characterised in [4], which contains a Walker A motif, and is structurally similar to the ATPase domain of ABC transporters. [1]. 9722634. Domain organization and functional analysis of Thermus thermophilus MutS protein [published erratum appears in Nucleic Acids Res 1998 Oct 15;26(20):following 4789]. Tachiki H, Kato R, Masui R, Hasegawa K, Itakura H, Fukuyama K, Kuramitsu S;. Nucleic Acids Res 1998;26:4153-4159. [2]. 8036718. Colon cancer and DNA repair: have mismatches met their match?. Jiricny J;. Trends Genet 1994;10:164-168. [3]. 8510668. The yeast gene MSH3 defines a new class of eukaryotic MutS homologues. New L, Liu K, Crouse GF;. Mol Gen Genet 1993;239:97-108. [4]. 11048710. Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA. Obmolova G, Ban C, Hsieh P, Yang W;. Nature 2000;407:703-710. (from Pfam)

Date:

2024-11-06

endonuclease MutS2 is a dsDNA-specific endonuclease/ATPase involved in the suppression of homologous recombination; it plays a role in the control of bacterial genetic diversity

Date:

2019-05-15