This is the C-terminal catalytic domain of tRNA pseudouridine synthase Pus10, conserved in archaea and eukaryotes [1-5]. In archaea it catalyses the formation of the pseudouridine at positions 54 and 55 in tRNA, while human Pus10 has different functions depending on its subcellular location: it is involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm [6,7]. Paper describing PDB structure 2apo. [1]. 16286935. The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs. Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR;. Nat Struct Mol Biol. 2005;12:1101-1107. Paper describing PDB structure 2aus. [2]. 16456033. Crystal structure determination and site-directed mutagenesis of the Pyrococcus abyssi aCBF5-aNOP10 complex reveal crucial roles of the C-terminal domains of both proteins in H/ACA sRNP activity. Manival X, Charron C, Fourmann JB, Godard F, Charpentier B, Branlant C;. Nucleic Acids Res. 2006;34:826-839. Paper describing PDB structure 2ey4. [3]. 16427014. Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita. Rashid R, Liang B, Baker DL, Youssef OA, He Y, Phipps K, Terns RM, Terns MP, Li H;. Mol Cell. 2006;21:249-260. Paper describing PDB structure 2hvy. [4]. 16943774. Crystal structure of an H/ACA box ribonucleoprotein particle. Li L, Ye K;. Nature. 2006;443:302-307. Paper describing PDB structure 2rfk. [5]. 18059286. Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex. Liang B, Xue S, Terns RM, Terns MP, Li H;. Nat Struct Mol Biol. 2007;14:1189-1195. [6]. 33023933. TRUNCATED at 1650 bytes (from Pfam)
- Date:
- 2024-10-16